The effects of mung bean seed-coat on glucose and lipid metabolism in diabetic mice by establishing a high-fat feeding combined with streptozotocin-induced diabetic mouse model was investigated. The results showed that， compared with the diabetic mice in the model group， a high-fat diet supplemented with mung bean seed-coat （6%） for 8 weeks could significantly reduced the blood glucose， blood lipid and glycated serum protein levels of mice in the intervention group （P<0.05）. Moreover， the insulin secretion levels and glucose tolerance were significantly increased and improved by mung bean seed-coat supplementation（P<0.05）， respectively. In addition， the hepatic and pancreatic histopathological observations showed that mung bean seed-coat supplementation could effectively relieve liver and pancreatic tissue damage and increase the number of islets β cells. The significant improvement effects of mung bean seed-coat on diabetic mice are of great practical significance for expanding its application and the development of auxiliary foods for reducing blood sugar.