陆生伊萨酵母WJL-G4降解柠檬酸的条件研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

哈尔滨市应用技术研究与开发项目(2017RAYXJ012)


Studies on the Conditions of Degradation of Citric Acid by Issatchenkia terricola WJL-G4
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:以本实验室分离获得的陆生伊萨酵母WJL-G4为试验菌株,在柠檬酸为唯一碳源的培养基中,考察该菌种的生长特性,降解柠檬酸最佳条件及其耐受性。方法:以降酸率和生长量为判断指标,对培养基中的柠檬酸质量浓度、氮源种类及添加量、无机盐种类及添加量进行筛选,并做单因素实验对温度、装液量、接种量、摇床转速、培养时间进行筛选,然后用正交设计试验优化菌种的降酸条件。结果:对培养基进行筛选,降酸最佳配方为柠檬酸质量浓度10 g/L,最佳氮源为酵母浸粉,添加量为5 g/L,最佳无机盐为硫酸镁,其最佳添加量为0.001 mol/L。在温度28℃、装液量40 mL/250 mL锥形瓶、接种量1%(体积分数)、转速160 r/min、时间12 h时,降酸率为91.82%,较优化前降酸效率提高4倍,且减少能源消耗,节约成本。此外,菌种可耐受SO2的最大质量浓度为8 mg/L,耐受的酒精体积分数为5%。结论:陆生伊萨酵母WJL-G4具有降解柠檬酸的功能,且在最佳降酸条件下其降酸效率显著提高。

    Abstract:

    Objective: The Issatchenkia terricola WJL-G4 isolated from raspberry orchard was used as the experimental strain. In a medium using citric acid as the sole carbon source, the growth characteristics of the strain, the best conditions for citric acid degradation, and its tolerance were investigated. Method: The deacidification and growth rate were used as judgment indicators to screen the citric acid concentration, nitrogen source type and addition amount, and inorganic salt type and addition amount in the culture medium, and the single factor test was used to screen the temperature, liquid loading, inoculation amount, shaking table speed and culture time, and then orthogonal design experiments were further used to optimize the acid reducing conditions of the strains. Result: The results showed that the medium was screened. The best formula for reducing acidity was 10 g/L of citric acid mass concentration, the best nitrogen source was yeast extract, the addition amount was 5 g/L, the best inorganic salt was magnesium sulfate, and the best addition amount was 0.001 mol/L. When the temperature was 28 ℃, the liquid volume was 40 mL/ 250 mL conical flask, the inoculation amount was 1%, the rotating speed was 160 r/min and the time was 12 h, the deacidification rate was 91.82%, which was not only four times more efficient before optimization, but also reduced energy consumption and saved costs. In addition, the maximum mass concentration of tolerant SO2 of the strain was 8 mg/L, and the alcohol volume fraction tolerated was 5%. Conclusion: The Issatchenkia terricola WJL-G4 had the function of degrading citric acid, and its acid reduction efficiency was significantly improved under the optimal acid reduction conditions.

    参考文献
    相似文献
    引证文献
引用本文

唐莹;王金玲;卢志全;蒋莹;何红英;董丹;晏雨辰;李巧月;.陆生伊萨酵母WJL-G4降解柠檬酸的条件研究[J].中国食品学报,2021,21(8):46-56

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-09-19
  • 出版日期:
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知