驼乳脂对CIA模型大鼠血清炎症因子及关节滑膜中炎症基因表达的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

内蒙古自然科学基金项目(2018BS03017,2020BS03011); 内蒙古自治区科技创新引导项目(KCMS2018048)


Effect of Camel Milk Fat on Serum Inflammatory Factors and Expression of Inflammatory Genes in Synovial Tissue of CIA Rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    探究驼乳脂对类风湿性关节炎大鼠模型的保护作用。以牛Ⅱ蛋白和弗氏完全佐剂的混合液注射于雌性Wistar大鼠皮下,构建Ⅱ型胶原诱导型关节炎(CIA)大鼠模型,成模后随机分为空白组、模型组、扶他林组和驼乳脂组。通过为期30 d的驼乳脂涂抹,测量大鼠体质量、后足肿胀度和脏器指数,观察滑膜切片病理学变化,大鼠关节骨表面变化、检测血清中炎性细胞因子质量浓度以及滑膜组织中重要基因mRNA的表达水平。经驼乳脂涂抹后,大鼠体质量明显增加;足爪肿胀显著降低(P<0.05);关节间隙和骨侵蚀明显恢复;滑膜组织中炎性细胞减少,新生细胞增加;显著提高了抗炎因子白介素-10(IL-10)的含量(P<0.05),显著降低了促炎因子白介素-1β(IL-1β)和白介素-6(IL-6)的含量(P<0.05);同时显著降低了滑膜组织中IL-1β、核转录因子-κB的抑制因子(IκB)、IκB激酶(IKK)、蛋白激酶1(RIP1) mRNA的表达量(P<0.05)。驼乳脂可通过抑制核转录因子-κB(NF-κB)通路降低炎症因子表达,从而缓解CIA大鼠关节炎。

    Abstract:

    To explore the protective effect of camel milk fat on rheumatoid arthritis in rats. A mixture of bovine protein II and Freund's complete adjuvant was injected subcutaneously into female Wistar rats to create CIA arthritis rat models. After modeling, the rats were randomly divided into blank group, model group, diclofenac sodium group and camel milk fat group. After 30 days of camel milk fat smearing treatment, the body weight, hind foot swelling, organ index, pathological changes of synovial slices and articular bone surface changes were measured. The content of inflammatory factors in serum were detected by ELISA. The expression of inflammatory genes in synovial tissues were detected by real-time fluorescence quantitative PCR. The results showed that the weight of rats was significantly increased(P<0.05), and the swelling of foot claws was significantly decreased(P<0.05). The pathological results showed that the joint clearance and bone erosion were significantly recovered, the inflammatory cells decreased, and the number of newborn cells increased in synovial tissue. The content of anti-inflammatory factor IL-10 (P<0.05) was significantly increased, and the content of inflammatory factor IL-1β and IL-6 was significantly reduced (P<0.05). The gene expression of IL-1β、IKK、IκB、RIP1 in synovial tissue was significantly reduced(P<0.05). Thus, camel milk fat might reduce inflammatory factor expression by inhibiting the NF-κB pathway, thereby alleviating arthritis in CIA rats.

    参考文献
    相似文献
    引证文献
引用本文

伊丽;李磊;王昆;佟臻昊;何静;斯仁达来;.驼乳脂对CIA模型大鼠血清炎症因子及关节滑膜中炎症基因表达的影响[J].中国食品学报,2021,21(8):111-120

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-09-19
  • 出版日期:
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知