小米提取物及其消化液的抗氧化和降糖作用
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家谷子糜子产业技术体系项目(CARS-07-13.5)


Antioxidant and Hypoglycemic Effects of Millet Extract and Its Digestive Solution
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    选用水、乙醇、正丁醇和正己烷为提取剂,测定小米不同溶剂提取物、提取物消化液及沉淀物消化液的DPPH·清除能力、总抗氧化能力,检测该类物质对高脂诱导的HepG2胰岛素抵抗细胞(IR-HepG2)糖吸收的影响。结果表明:水提物及乙醇提取物有较高的抗氧化活性,经体外模拟消化后其抗氧化能力降低(总抗氧化能力分别降低50.00%和54.55%)。与抗氧化结果不同,水提物及乙醇提取物(20 μg/mL和40 μg/mL)对IR-HepG2胰岛素抵抗改善作用不显著,而正丁醇提取物(40 μg/mL和80 μg/mL)及正己烷提取物(20,40,80 μg/mL)均对IR-HepG2胰岛素抵抗有显著地改善作用。经消化,乙醇提取物对胰岛素抵抗的改善作用显著增强,正丁醇和正己烷提取物对胰岛素抵抗的改善作用无明显变化。正己烷沉淀物消化液、正丁醇沉淀物消化液、水沉淀物消化液及小米消化液均可提高IR-HepG2的糖吸收,而乙醇沉淀物对细胞的胰岛素抵抗并无改善作用,说明小米中起到降糖作用的活性物质主要集中在乙醇提取物中。

    Abstract:

    In order to explore the anti-oxidation and hypoglycemic activity of millet, the DPPH· scavenging capacity and total antioxidant capacity of four different extracts, extracts digestive solution and sediment digestive solution of millet were determined. The glucose uptake levels on PA-induced insulin-resistant HepG2 cells (IR-HepG2) were detected. The results showed that the water extract and ethanol extract had higher antioxidant activity, and their antioxidant capacity decreased after simulated digestion in vitro (the total antioxidant capacity decreased by 50.00% and 54.55%, respectively). Unlike the antioxidant results, the water extract and the ethanol extract (20, 40 μg/mL) had no significant effect on the improvement of IR-HepG2 insulin resistance. The n-butanol extract (40, 80 μg/mL) and n-hexane extract (20, 40, 80 μg/mL) all significantly increased the glucose uptake of IR-HepG2. After digestion, the ethanol extract significantly improved insulin resistance, but n-butanol and n-hexane extracts had no effect on insulin resistance. The glucose uptake was enhanced by n-hexane sediment digestive solution, n-butanol sediment digestive solution, water sediment digestive solution and millet digestive solution. However, ethanol sediment digestive solution did not improve insulin resistance, indicating that the hypoglycemic effect of millet was mainly concentrated in ethanol extract.

    参考文献
    相似文献
    引证文献
引用本文

尹瑞旸;李星;胡锦荣;胡小松;沈群.小米提取物及其消化液的抗氧化和降糖作用[J].中国食品学报,2021,21(11):14-21

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-12-17
  • 出版日期:
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知