活菌饮料中乳酸菌活菌数的光学快速定量检测
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“十三五”国家重点研发计划项目(2016YFD0101205)


Optical Rapid Quantitative Detection of the Viable Number of Lactic Acid Bacteria in Living Bacteria Beverage
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    摘要:

    利用傅里叶变换近红外分析仪与紫外-可见分光光度计,以市售活菌饮料为研究对象,进行饮料中乳酸菌活菌数的快速定量检测。对活菌饮料的原始光谱分别进行Savitzky-Golay(SG)平滑结合一阶求导(FD)、多元散射校正(MSC)及正交信号校正(OSC)预处理,利用偏最小二乘法建立乳酸菌活菌数定量预测模型,并对最优模型进行优化与外部验证。结果表明:对于活菌饮料中乳酸菌活菌数,采用OSC预处理的傅里叶变换近红外光谱建立的PLS模型效果最好。利用竞争自适应重加权法(CARS)进行波长筛选与模型的优化,校正集相关系数(Rc)为0.9974,校正集均方根误差(RMSEC)为0.0211,验证集相关系数(Rp)为0.9837,验证集均方根误差(RMSEP)为0.0508。利用未参与建模的市售样品对优化后的傅里叶变换近红外预测模型进行外部验证,预测值相关系数为0.9068,均方根误差为0.0108。结果表明:傅里叶变换近红外光谱能够快速、准确地检测活菌饮料中乳酸菌数活菌数,为活菌饮料生产销售流通中的质量监测提供一种快捷方便的方法。

    Abstract:

    In this paper, using a Fourier transform near-infrared analyzer and an ultraviolet-visible spectrophotometer, with a commercial active beverage as a research object, a rapid quantitative detection study on the number of live lactic acid bacteria in live bacterial beverage was carried out. The original spectra of live bacterial beverages were subjected to Savitzky-Golay (SG) smoothing combined with first-order derivation (FD), multivariate scattering correction (MSC) and orthogonal signal correction(OSC) pretreatment, and then using partial least squares to establish lactic acid bacteria a quantitative prediction model for the number of viable bacteria, and optimization and external verification of the optimal model. For the number of live lactic acid bacteria in live bacterial beverages, the PLS model established by Fourier transform near infrared spectroscopy with OSC pretreatment was the best. Using competitive adaptive weighting method (CARS) for wavelength screening and model optimization, the correction set correlation coefficient and root mean square error Rc was 0.9974, the RMSEC was 0.0211, the verification set correlation coefficient Rp was 0.9837, the root mean square error RMSEP was 0.0508. Finally, using the commercial samples that did not participate in the modeling, the optimized Fourier transform near infrared prediction model was externally verified. The correlation coefficient of the predicted value was 0.9068, and the root mean square error was 0.0108. The results showed that based on Fourier transform near infrared spectroscopy, the number of lactic acid bacteria in live bacterial beverages could be quickly and accurately detected, which provided a quick and convenient method for quality monitoring in the production and sales of live bacterial beverages.

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王绮;李永玉;彭彦昆;韩东海;刘亚超.活菌饮料中乳酸菌活菌数的光学快速定量检测[J].中国食品学报,2021,21(11):236-243

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  • 在线发布日期: 2021-12-17
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