In this paper， a fluorescent molecularly imprinted polymers with high selective recognition sites for octopamine was synthesized using octopamine as temple， upconversion fluorescent nanoparticles as matrix and the element of fluorescent sensing by a surface imprinting technique in combination with a sol-gel process. The developed polymers were applied to the quantitative determination of octopamine. Under the optimum conditions， the fluorescence intensity of polymers increased linearly with the increasing concentration of octopamine ranging 2-10 mg/L with a detection limit of 0.37 mg/L（S/N=3）. In order to validate the applicability， the fluorescent method was used to detect octopamine in complex samples including wine and cheese. The recoveries ranging 86% - 98% with a relative standard deviation（RSD） ranging from 0.54% to 4.65% were obtained. The above result indicated that the proposed fluorescent method provided a new platform for the measurement of octopamine in food samples.