Based on the macrophage and adipocytes co-cultured system， effect of catechins on the gene expression of cytokines and inflammatory enzymes， the phosphorylation levels of AMPK and SIRT1 was studied， and to preliminarily research the anti-inflammatory mechanism of catechin. Catechin with the mass concentrations of 0， 50， 100 μg/mL were added to the four treatments， respectively. The gene expression of cytokines and inflammatory enzymes， phosphorylation levels of AMPK and SIRT1 in adipocytes were determined by quantitative RT-PCR and Western blotting， respectively. Catechin obviously inhibited the gene expression level of pro-inflammatory cytokines （IL-1， IL-6， IL-12） and inflammatory enzymes（iNOS， COX-2）， but promoted the expression of anti-inflammatory cytokines（IL-4， IL-10）. High mass concentration of catechin increased the phosphorylation level of AMPK and SIRT1， it is synergistic between AMPK and SIRT1. In the macrophage and adipocytes co-cultured system， the secretion of TNF-α in macrophages fully induced the differentiation of adipocytes. Catechin has an anti-inflammatory effect by regulating the gene expression of cytokines via AMPK/SIRT1 signaling pathway.