甲鱼抗氧化肽对非酶糖基化反应及其终产物的抑制作用

doi:10.16429/j.1009-7848.2022.02.007

2025年4月6日 14:46 星期日

首页 > 过刊浏览>2022年第22卷第2期 >58-64. DOI:10.16429/j.1009-7848.2022.02.007

甲鱼抗氧化肽对非酶糖基化反应及其终产物的抑制作用
DOI:
                        10.16429/j.1009-7848.2022.02.007
                    
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作者:
                        王楠1王楠
浙江树人学院生物与环境工程学院 杭州 310015
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章涵钰1章涵钰
浙江树人学院生物与环境工程学院 杭州 310015
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楼博1楼博
浙江树人学院生物与环境工程学院 杭州 310015
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王伟2王伟
浙江省农业科学院农产品质量标准化研究所 杭州 310021
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史学波3史学波
浙江中得农业集团有限公司 杭州 310021
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作者单位:(1.浙江树人学院生物与环境工程学院 杭州 310015;2.浙江省农业科学院农产品质量标准化研究所 杭州 310021;3.浙江中得农业集团有限公司 杭州 310021)
作者简介:王楠(1981—)，女，博士，教授 E-mail: xiaofeiyu0708@163.com
通讯作者:
中图分类号:
基金项目:浙江省自然科学基金项目(LY17C200012)

Inhibitory Effect of Antioxidant Peptides of Turtle on Non-enzymatic Gluco-sylation and Its End Products

Author:

Wang Nan ^¹
Wang Nan
College of Biology and Environmental Engineering， Zhejiang Shuren University， Hangzhou 310015
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Zhang Hanyu ^¹
Zhang Hanyu
College of Biology and Environmental Engineering， Zhejiang Shuren University， Hangzhou 310015
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Lou Bo ^¹
Lou Bo
College of Biology and Environmental Engineering， Zhejiang Shuren University， Hangzhou 310015
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Wang Wei ^²
Wang Wei
Institute of Quality and Standard for Agricultural Products， Zhejiang Academy of Agricultural Sciences，Hangzhou 310021
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Shi Xuebo ^³
Shi Xuebo
Zhejiang Zhongde Agriculture Group Co.Ltd.， Hangzhou 310021
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Affiliation:

(1.College of Biology and Environmental Engineering， Zhejiang Shuren University， Hangzhou 310015;2.Institute of Quality and Standard for Agricultural Products， Zhejiang Academy of Agricultural Sciences，Hangzhou 310021;3.Zhejiang Zhongde Agriculture Group Co. Ltd.， Hangzhou 310021)

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摘要

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参考文献 [18]

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摘要:

建立体外非酶糖基化蛋白质-还原糖模拟反应体系的基础上，探究甲鱼抗氧化肽对非酶糖基化反应的抑制率(NEG)和晚期糖基化终末产物(AGEs)抑制率的影响。结果表明:甲鱼抗氧化肽能够抑制非酶糖基化反应和晚期糖基化终产物的产生，具有剂量依赖性。当甲鱼抗氧化肽处理蛋白质-还原糖体系21 d时，1.00 mg/mL甲鱼抗氧化肽对NEG的抑制率达68.79%；当处理28 d时，1.00 mg/mL甲鱼抗氧化肽对AGEs的抑制率达89.35%。红外光谱结果也证实了甲鱼抗氧化肽能够抑制糖基化反应进程。

关键词:甲鱼抗氧化肽; 非酶糖基化反应; 晚期糖基化终末产物; 抑制作用

Abstract:

On the basis of establishing the simulated reaction system of non-enzyme gluco-sylation protein and reducing sugar in vitro， the inhibitory effect of turtle antioxidant peptide on non-enzyme gluco-sylation(NEG) reaction and advanced glycation end products (AGEs) was investigated. The results showed that the mass concentration of anti-oxidation peptide was 1.00 mg/mL， and the inhibition rate of NEG was 68.79% when incubated for 21 days， the inhibition rate of AGEs was 89.35% when incubated for 28 days. Infrared spectroscopy results proved that antioxidant peptide of turtle could effectively inhibit the production of AGEs in non-enzymatic gluco-sylation reaction.

Key words:turtle antioxidant peptide; non-enzymatic gluco-sylation reaction; advanced glycation end products; inhibitory effect

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