To study the effects of lotus seedpod oligomeric procyanidins(LSOPC) on tissue damage and intestinal microflora disorder in mice induced by advanced glycation end products (AGEs)， healthy male mice as animal model were divided into 4 groups and fed 12 weeks with high AGEs AIN-93G， high AGEs AIN-93G added 0.2% or 0.5% LSOPC (w/w)， and normal AIN-93G as the control group. The effects of AGEs and LSOPC on mice weight， diet， water intake， organs indexs， intestinal tissue damage， colonic tight junction proteins and oligopeptide transporter expression， intestinal inflammatory factor and intestinal microflora disorder were analysis. Our results showed that both 0.2% LSOPC and 0.5% LSOPC had obvious ameliorative effects on tissue injury， increased the expression of ZO-1 and occludin， and decreased the expression of Pept1， TNF-α， IL-6， VCAM-1 and ICAM-1. Intestinal environment and intestinal microbial disorder were also significantly improved， which were dose-dependent on LSOPC. Conclusion: LSOPC could reduce mice tissue damage and intestinal microflora disorder caused by dietary AGEs， and has the potential to develop into a basic dietary supplement.