16种市售茶叶抗氧化活性及抗氧化物质分析
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(江苏大学食品与生物工程学院 江苏镇江 212013)

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冯拓(1996—),男,硕士生

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国基自然科学基金项目(31801537);中山市重大科技专项(2018A1007);阳西市科技创新战略专项(SDZX2021030)


Analysis of Antioxidant Activities and Antioxidant Substances of Sixteen Kinds of Commercial Tea
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(School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu)

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    摘要:

    以16种市售销量较好的茶叶为代表,对比分析不同茶叶的茶汤中的总酚、总黄酮、氨基酸态氮、还原糖和总糖等抗氧化物质含量,并以抗坏血酸为阳性对照,采用2,2-联苯基-1-苦基肼基(DPPH)自由基清除能力、2,2'-联氨-双二胺盐(ABTS)自由基清除能力、还原力和金属离子螯合能力等抗氧化指标评价茶汤的抗氧化活性。采用相关性分析、聚类分析和主成分分析等方法揭示茶汤的抗氧化物质与抗氧化活性之间的复杂关系。结果表明,除少数茶汤样品间无显著性差异(P>0.05)外,其余不同茶汤的抗氧化物质含量和抗氧化活性差异显著(P<0.05)。其中金坛雀舌和黄金芽的DPPH自由基清除能力最强,西湖龙井的ABTS自由基清除能力最强,黄金芽的还原力最强,南京黄龙岘的金属螯合力最强,黄金芽、南京黄龙岘、金坛雀舌、西湖龙井、信阳毛尖综合评分较高。对市售茶叶抗氧化活性和抗氧化物质的综合评价,可为茶叶抗氧化机制研究提供基础数据,同时为消费者有针对性地选择茶叶提供参考。

    Abstract:

    In this study, 16 kinds of teas with good sales were selected as the representative, the contents of total phenols, total flavonoids, amino acid nitrogen, reducing sugar and total sugar in different tea samples were compared, and ascorbic acid was used as the positive control, scavenging activity against DPPH radical, scavenging activity against ABTS radical, reducing power and metal ion chelating activity were used to evaluate the antioxidant activity of different tea samples; in addition, correlation analysis, cluster analysis and principal component analysis were used to reveal the complex relationship between antioxidant substances and antioxidant activities of 16 kinds of tea samples. The results showed that there were significant differences in the contents of antioxidant substances and antioxidant activities among different tea samples except a few tea samples, the scavenging activity against DPPH radical of the Buxus jintan tea and Golden sprout tea were the strongest, the scavenging activity against ABTS radical of West Lake Longjing tea was the strongest, the reducing power of Golden sprout tea was the strongest, the metal ion chelating activity of Nanjing Huanglongxian tea was the strongest. Golden sprout tea, Nanjing Huanglongxian tea, Buxus jintan tea, West Lake Longjing tea and Xinyang Maojian tea had higher comprehensive scores. In this study, the antioxidant activities and antioxidant substance of commercially available teas were evaluated comprehensively, which can lay data foundation for the study of antioxidant mechanism of teas and provide reference for consumers to select tea.

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冯拓,单培,张展开,高献礼,马海乐,王博.16种市售茶叶抗氧化活性及抗氧化物质分析[J].中国食品学报,2022,22(10):352-362

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  • 收稿日期:2021-10-11
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  • 在线发布日期: 2022-11-24
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