盐度对紫甘蓝自然发酵过程中细菌多样性及品质的影响
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(东北农业大学食品学院 哈尔滨 150030)

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Effects of Salt Concentration on the Microbial Diversity and Quality of Red Cabbage during Natural Fermentation
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(College of Food Science and Technology, Northeast Agricultural University, Harbin 150030)

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    摘要:

    以不同盐度水平(1%,3%,5%,10%)下自然发酵的紫甘蓝为研究对象,利用16S rRNA扩增子测序技术分析其自然发酵过程中细菌多样性的变化规律,同时对其自然发酵过程中理化特性的变化及感官品质进行研究。结果表明:4种盐度下自然发酵的紫甘蓝酸菜均以变形菌门和厚壁菌门为主,主要优势菌属为乳杆菌属、肠杆菌属。除此之外,1%盐度下自然发酵的紫甘蓝酸菜的优势菌属还有乳球菌属,而3%盐度有泛菌属,5%和10%盐度则有明串珠菌属。不同盐度下自然发酵的紫甘蓝酸菜在自然发酵过程中pH值随发酵时间呈下降趋势,总酸均随发酵时间增加,还原糖含量和亚硝酸盐含量则随发酵时间呈先升高后下降的趋势。相关性分析显示,乳杆菌属、肠杆菌属、乳球菌属等菌属与理化特性的相关性较大。综上,3%盐度下自然发酵的紫甘蓝酸菜样品细菌多样性丰度较高,总酸累积最高,且感官品质较好,较适合紫甘蓝的发酵。

    Abstract:

    In this study, the changes in microbial diversity during natural fermentation of red cabbage at different salt concentration levels (1%, 3%, 5% and 10%) were investigated by using 16S rRNA amplicon sequencing technology, and the changes in physicochemical properties during natural fermentation of red cabbage were determined, as well as the sensory evaluation of naturally fermented red cabbage at different salt concentrations was also carried out. The results showed that the red cabbage naturally fermented under different salt concentrations was dominated by Proteobacteria and Firmicutes, and the main genera are Lactobacillus, Enterobacter, and Lactococcus, Leuconostoc, Serratia, Weissella, etc. In addition, different salt concentrations had significant effects on the physicochemical properties of fermented red cabbage such as pH value, total acid, reducing sugars and nitrite, among which the fermented red cabbage at 3% salt concentration had the highest accumulation of total acid, faster consumption rate of reducing sugars and relatively faster decrease rate of nitrite. The correlation results showed that Lactobacillus, Enterobacter, Lactococcus, Weissella, Streptococcus, Akkermansia and pH value, total acid, reducing sugar, nitrite and other physical and chemical properties were highly correlated. According to the sensory evaluation, it was concluded that naturally fermented red cabbage samples at 3% salt concentration had the best taste, followed by 1% and 5% salt concentration, and 10% salt concentration had poor taste. Therefore, red cabbage samples naturally fermented at 3% salt concentration had higher microbial diversity abundance, highest accumulation of total acid, and better taste and flavor, which was more suitable for the fermentation of red cabbage.

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柳凯,解双瑜,李智,孙波.盐度对紫甘蓝自然发酵过程中细菌多样性及品质的影响[J].中国食品学报,2022,22(11):379-389

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  • 收稿日期:2021-11-05
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  • 在线发布日期: 2022-12-28
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