In order to develop and utilize Clarias fuscus skin， conventional nutrient analysis methods， gas chromatography-mass spectrometry (GC-MS)， gas chromatography-ion mobility spectroscopy (GC-IMS) technology were used to analyze the nutrients and volatile substances in fish skin. The results showed that the water content， crude protein， crude fat， crude ash and total sugar content in fish skin were 62.85%， 27.50%， 5.28%， 0.15%， 3.84%， respectively. The essential amino acid content in fish skin was 16.60 g/100 g， the umami amino acid content was 45.72 g/100 g. The content of essential free amino acid and fresh free amino acid were 52.6 mg/100 g and 30.54 mg/100 g， respectively. Taurine had the highest free amino acid content， which was 28.34 mg/100 g. The content of unsaturated fatty acids in the skin was relatively rich and the oleic acid was the highest fatty acid content that was 1.18 mg/mL. Many mineral elements were contained in fish skin. Trans-2-octenal， benzaldehyde， n-hexanal and n-hexanol could be obtained by GC-MS and GC-IMS analysis methods. Therefore， the combination of GC-MS and GC-IMS analysis methods could better study the flavor substances of catfish skin.