海洋鱼低聚肽螯合铁的小肠吸收特性研究

doi:10.16429/j.1009-7848.2023.04.011

2025年4月6日 18:31 星期日

首页 > 过刊浏览>2023年第23卷第4期 >106-115. DOI:10.16429/j.1009-7848.2023.04.011

海洋鱼低聚肽螯合铁的小肠吸收特性研究
DOI:
                        10.16429/j.1009-7848.2023.04.011
                    
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作者:
                        泮雯霏1泮雯霏
浙江大学医学院公共卫生系 杭州 310000
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应晓玲1应晓玲
浙江大学医学院公共卫生系 杭州 310000
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马倩2马倩
浙江大学化学系 杭州 310000
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徐玉英1徐玉英
浙江大学医学院公共卫生系 杭州 310000
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李宁2李宁
浙江大学化学系 杭州 310000
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杨敏1杨敏
浙江大学医学院公共卫生系 杭州 310000
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作者单位:(1.浙江大学医学院公共卫生系 杭州 310000;2.浙江大学化学系 杭州 310000)
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基金项目:浙江省基础公益研究计划项目(LGF18H260009) 第一作者: 泮雯霏，女，硕士生

Studies on the Intestinal Absorption of Marine Fish Oligopeptide Chelated Iron

Author:

Pan Wenfei ^¹
Pan Wenfei
Department of Public Health， Zhejiang University School of Medicine， Hangzhou 310000
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Ying Xiaoling ^¹
Ying Xiaoling
Department of Public Health， Zhejiang University School of Medicine， Hangzhou 310000
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Ma Qian ^²
Ma Qian
Zhejiang University Department of Chemistry， Hangzhou 310000
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Xu Yuying ^¹
Xu Yuying
Department of Public Health， Zhejiang University School of Medicine， Hangzhou 310000
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Li Ning ^²
Li Ning
Zhejiang University Department of Chemistry， Hangzhou 310000
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Yang Min ^¹
Yang Min
Department of Public Health， Zhejiang University School of Medicine， Hangzhou 310000
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Affiliation:

(1.Department of Public Health， Zhejiang University School of Medicine， Hangzhou 310000;2.Zhejiang University Department of Chemistry， Hangzhou 310000)

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摘要:

目的:探究海洋鱼低聚肽螯合铁(MCOP-Fe)的小肠吸收特性，并与传统补铁剂硫酸亚铁(FeSO4)进行比较。方法:建立体外大鼠外翻肠囊模型，选取雌性Sprague Dawley大鼠40只，随机分为8组【低、中、高剂量(3，5，10 μg/mL)MCOP-Fe组；低、中、高(3，5，10 μg/mL)FeSO4组；10 μg/mL MCOP-Fe+500 μg/mL植酸组；10 μg/mL FeSO4+500 μg/mL植酸组】，用肠道单位面积铁累计吸收率Q评价不同肠段吸收铁的效力。结果:1)中、低、高浓度MCOP-Fe组在十二指肠、空肠及回肠的Q值随浓度呈现梯度增加，而FeSO4组没有类似的趋势；2)中、低浓度FeSO4组的Q值几乎都有高于MCOP-Fe组的趋势，而在高浓度时相反；3)添加500 μg/mL植酸后，高浓度MCOP-Fe组和FeSO4组Q值均有下降，高浓度FeSO4组下降更为显著(P<0.05)。结论:在3~10 μg/mL剂量范围内，FeSO4的小肠吸收率呈现饱和的平衡趋势，而MCOP-Fe呈现梯度上升的趋势；同时MCOP-Fe受植酸的抑制作用小，相对FeSO4更加稳定，应用前景良好。

关键词:外翻肠囊法; 海洋鱼; 低聚肽螯合铁; 小肠吸收; 铁缺乏

Abstract:

Objective: To explore the absorption characteristics of marine fish oligopeptide chelated iron (MCOP-Fe) in small intestine， and compare it with the traditional iron supplement-ferrous sulfate (FeSO4). Methods: 40 female Sprague Dawley rats were randomly divided into 8 groups (L-MCOP-Fe: 3 μg/mL MCOP-Fe； M-MCOP-Fe: 5 μg/mL MCOP-Fe； H-MCOP-Fe: 10 μg/mL MCOP-Fe； L-FeSO4: 3 μg/mL FeSO4； M- FeSO4: 5 μg/mL FeSO4； H-FeSO4: 10 μg/mL FeSO4； H-MCOP-Fe+PA: 10 μg/mL MCOP-Fe+500 μg/mL phytic acid； H-FeSO4+PA: 10 μg/mL FeSO4 + 500 μg/mL phytic acid). To establish the model of everted gut sac in vitro， the effect of iron absorption in different intestinal segments was evaluated by the cumulative iron absorption rate per unit area(Q). Results: 1) The Q values of duodenum， jejunum and ileum in three MCOP-Fe groups increased with concentration gradient， but there was no similar trend in FeSO4 groups； 2) The Q values of group L-FeSO4 and M-FeSO4 had a tendency to be higher than those of group L-MCOP-Fe and M-MCOP-Fe， but were opposite at high concentration (10 μg/mL)； 3) After adding 500 μg/mL phytic acid， the Q values of groups H-MCOP-Fe+PA and H-FeSO4+PA decreased compared with groups H-MCOP-Fe and H-FeSO4， and the decrease degree of group H-FeSO4+PA was more significant (P<0.05). Conclusion: In the range of 3-10 μg/mL， the absorption rate of FeSO4 in small intestine showed an equilibrium trend of saturation， while the MCOP-Fe showed a gradient trend of increase； at the same time， MCOP-Fe was less inhibited by phytic acid， and was more stable than FeSO4， which has good application prospects.

Key words:everted gut sac; marine fish; ferrous oligopeptide chelata; intestinal absorption; iron-deficiency

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