(黑龙江中医药大学药学院 哈尔滨 150040)
(School of Pharmacy， Heilongjiang University of Chinese Medicine， Harbin 150040)
为探讨复合益生菌对环磷酰胺诱导免疫低下模型小鼠免疫功能的影响，将BALB/c小鼠随机分为空白组和模型组(灌胃生理盐水)，阳性对照组【灌胃盐酸左旋咪唑(10 mg/kg)】，低、中、高剂量实验组(分别灌胃2.5×106，5×106，1×107 CFU/0.4 mL复合益生菌)，持续实验28 d，测定小鼠脏器指数、细胞免疫功能、体液免疫功能、脾淋巴细胞亚群百分比和血清免疫球蛋白及细胞因子水平。结果表明，与模型组比较，各剂量组小鼠胸腺指数明显增加(P＜0.01)，低、中、高剂量实验组分别增加58.92%，67.86%，80.36%；迟发型变态反应程度增加(P＜0.01)，低、中、高剂量实验组分别增加20.34%，27.12%，67.80%；血清溶血素水平均增加(P＜0.05)，以中剂量和高剂量组增加显著(P＜0.01)，分别增加31.25%，40.23%；低、中、高剂量实验组脾淋巴细胞CD3+、CD4＋CD8-和CD4-CD8＋百分比增加(P＜0.05)，以CD3+最为显著(P＜0.01)，低、中、高剂量实验组分别增加33.17%，42.63%，54.65%；高剂量组自然杀伤(NK)细胞比例明显增加56.50%(P＜0.01)，血清免疫球蛋白IgA、IgG、IL-2、TNF-α水平均升高(P＜0.05)。结论:复合益生菌对环磷酰胺诱导小鼠免疫力低下具有显著改善作用，为增强免疫力作用的保健食品开发提供了参考依据。
In order to investigate the effect of complex probiotics on immune function of cyclophosphamide-induced immunosuppression model mice， BALB/c mice were randomly divided into blank group and model group (perfused with normal saline)， positive control group [perfused with levamisole hydrochloride (LEV， 10 mg/kg)]， and low-dose， medium-dose， high-dose experimental group (perfused with 2.5×106， 5×106， 1×107 CFU/0.4 mL composite probiotics) for 28 days. The viscera index， cellular immune function， humoral immune function， percentage of splenic lymphocyte subsets， serum immunoglobulin and cytokine levels were determined. The results showed that， compared with model group， the thymus index of mice in each dose group was significantly increased (P<0.01)， and that in the low， medium and high dose groups was increased by 58.92%， 67.86% and 80.36%. The degree of delayed type allergic reaction increased (P<0.01) 20.34%， 27.12% and 67.80% in low， medium and high dose groups. Serum hemolysin levels were increased in both groups (P<0.05)， and and were significantly increased in medium dose and high dose groups (P<0.01)， by 31.25% and 40.23%， respectively. The percentages of CD3+， CD4+CD8- and CD4-CD8+ in splenic lymphocytes increased in low， medium and high dose groups (P<0.05)， and CD3+ was the most significant (P<0.01)， while those in low， medium and high dose groups increased by 33.17%， 42.63% and 54.65%， respectively. The proportion of natural killer (NK) cells in high-dose group was significantly increased by 56.50% (P<0.01)， and serum levels of immunoglobulin IgA， IgG， IL-2 and TNF-α were increased (P<0.05). In conclusion， compound probiotics could significantly improve the immunity of CTX mice， providing an important reference for the development of health food to enhance the immunity.