不同工艺制取油莎豆油的品质与风味分析
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(1.中国农业科学院油料作物研究所 武汉 430062;2.武汉轻工大学 武汉 430023;3.油料油脂加工技术国家地方联合工程实验室 武汉 430062)

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中国农业科学院科技创新工程(CAAS-ASTIP-2021-OCRI);支持企业技术创新发展项目(2021BLB151)


Analysis of the Quality and Flavor of Tiger Nut Oil Produced by Different Oil Extraction Methods
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(1.Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062;2.Wuhan Polytechnic University, Wuhan 430023);3.Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Wuhan 430062)

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    摘要:

    分别采用液压榨(HP)、螺旋压榨(SP)、亚临界丁烷萃取(SBE)和超临界CO2萃取(SCE)法提取油莎豆油,研究4种工艺对出油率、油脂理化指标和脂质伴随物含量的影响,并通过顶空固相微萃取-气质联用法分析4种油莎豆油的挥发性成分。结果表明:制油工艺对油莎豆油出油率及基本理化指标的影响显著(P<0.05),SBE出油率最高(31.89%),在SCE、SP、SP油样中分别检测出最低的酸价(0.44 mg/g)、过氧化值(0.03×10-1 g/100 g)和水分(0.06 g/100 g)。SCE油和SBE油的色泽更加清亮透明。SCE油中不饱和脂肪酸含量为49.63 mg/g,比其它3种工艺增加了3.50%~5.54%(P<0.05)。4种油莎豆油样中脂质伴随物含量及其氧化稳定性有显著差异(P<0.05),SP油中生育酚(26.85 mg/100 g)、总酚(16.65 mg GAE/100 g)含量最高,SCE油中总甾醇(283.48 mg/100 g)含量最高,SBE油中磷脂含量(1.05 mg/g)最高。SP油表现出较强的氧化稳定性,其氧化诱导期为34.40 h,分别是SBE、HP和SCE油的1.24,8.27倍和6.70倍。从4种油莎豆油中共检出162种挥发性物质,主要为杂环类物质、酸类、醛类、醇类、酮类、酯类和烃类,SP油中杂环类相对含量最高为44.86%,HP油及SBE油中醛类物质相对含量最高分别为50.18%和26.03%,SCE油中苯类物质相对含量最多为60.00%。根据气味活力值(OAV)得出4种油样的关键风味物质,其中SP油有16种,HP油有23种,SCE油有8种,SBE油有22种,己醛为共有成分。主成分分析(PCA)发现4种油的特征风味存在差异。研究结果可为油莎豆油的产品开发提供数据参考。

    Abstract:

    Hydraulic pressing (HP), spiral pressing (SP), subcritical butane extraction (SBE), and supercritical CO2 extraction(SCE) were used to extract oil from tiger nuts. The effects of four oil extraction methods on the oil yield, physicochemical indicators, and lipid concomitant content of tiger nut oil were systematically compared. The volatile components of the four types of oil were analyzed by headspace solid-phase microextraction gas chromatography-mass spectrometry. The results showed that different oil extraction methods significantly impacted tiger nut oil's oil yield and basic physicochemical indicators (P<0.05). SBE had the highest oil yield (31.89%), and the lowest acid value (0.44 mg/g), peroxide value (0.03×10-1g/100 g), and water content (0.06 g/100 g) were detected in SCE, SP, and SP oil samples, respectively; The color of tiger nut oil extracted by SCE and SBE were clearer and more transparent. The content of unsaturated fatty acid in SCE oil was 49.63 mg/g, which was significantly increased by 3.50%-5.54% (P<0.05) compared with the other three methods. There were significant differences (P<0.05) in the content of lipid concomitant and oxidative stability among four tiger nut oil samples (P<0.05). The content of tocopherols (26.85 mg/100 g) and total phenols (16.65 mg GAE/100 g) in SP oil was the highest, the content of total sterols (283.48 mg/100 g) in SCE oil was the highest, and the content of phospholipids (1.05 mg/g) in SBE oil was the highest. SP oil exhibits strong oxidative stability, the oxidation induction period was 34.40 hours, which was 1.24 times, 8.27 times, and 6.70 times that of SBE oil, HP oil, and SCE oil, respectively. A total of 162 volatile compounds were detected in the four oil samples, mainly including heterocyclic compounds, acids, aldehydes, alcohols, ketones, esters, and hydrocarbons. The relative content of heterocyclic compounds in SP oil was the highest at 44.86%, and the relative content of aldehydes in HP oil and SBE oil was the highest at 50.18% and 26.03%, respectively, the maximum relative content of benzene compounds in SCE oil was 60.00%. According to OAV, key flavor compounds were identified for four oil samples, including 16 for SP oil, 23 for HP oil, 8 for SCE oil, and 22 for SBE oil. Among them, hexanal was the common component; PCA found differences in the characteristic flavors of four oil samples. The research results can provide a theoretical reference for the development of tiger nut oil products.

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邓淑君,杨国燕,刘佳杰,郑明明,万楚筠.不同工艺制取油莎豆油的品质与风味分析[J].中国食品学报,2024,24(9):443-456

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  • 收稿日期:2023-09-26
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  • 在线发布日期: 2024-10-21
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