宁夏不同产地枸杞叶生物碱组成与活性差异
作者:
作者单位:

(宁夏大学食品科学与工程学院 银川 750021)

基金项目:

国家自然科学基金项目(32160534);宁夏重点研发项目(2022BBF02006)


The Differences in Alkaloid Composition and Activity of Goji Leaves from Different Regions of Ningxia
Author:
Affiliation:

(School of Food Science & Engineering, Ningxia University, Yinchuan 750021)

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [30]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    以宁夏3个产地(银川、中宁、固原)“宁杞菜1号”枸杞叶为材料,基于植物广泛靶向代谢组技术研究其生物碱组成和体外抗氧化活性差异。结果表明,3个产地样品的生物碱提取物均具有体外抗氧化活性。其中,对ABTS+的清除能力,中宁(35.4%)>银川(29.6%)>固原(28.9%);对超氧阴离子清除能力,银川(84.8%)>固原(83.9%)>中宁(82.1%);总还原能力中宁(0.46)>固原(0.45)>银川(0.41)。3个产地共检出12类162种生物碱代谢物,在差异倍数≥2或≤0.5,P<0.05,VIP≥1的筛选条件下,银川和固原比较组中显著变化的生物碱差异代谢物有53种(其中上调21种,下调32种),中宁和固原比较组中显著变化的差异代谢物有25种(其中上调16种,下调9种),银川和固原比较组中显著变化的差异代谢物有59种(其中上调31种,下调28种)。3个产地同时比较,筛选出差异显著的生物碱代谢物有5种(二氢咖啡酰腐胺、泛酰巯基乙胺、枸杞素D、对乙酰氨基酚、3,4-二甲氧基苯甲酰胺),KEGG数据库通路富集分析发现5种差异显著生物碱代谢物主要分布在4条代谢途径中,分别为代谢途径、次生代谢产物的生物合成、碳青霉烯生物合成、泛酸盐和辅酶A生物合成途径。本研究为宁夏不同产地“宁杞菜1号”枸杞叶的营养物质挖掘提供理论基础。

    Abstract:

    In this study, the differences in alkaloid composition and antioxidant activities in vitro were investigated based on plant wide targeted metabolomic techniques using the ‘Ningqicai No. 1’ goji leaves from three origins in Ningxia (Yinchuan, Zhongning, and Guyuan). The results showed that the alkaloid extracts of the samples from the three origins all had in vitro antioxidant activities. Among them, the scavenging ability of ABTS+ is Zhongning (35.4%) > Yinchuan (29.6%) > Guyuan (28.9%). The scavenging ability of superoxide anion is Yinchuan (84.8%) > Guyuan (83.9%) > Zhongning (82.1%). The total reduction capacity is Zhongning (0.46) > Guyuan (0.45) > Yinchuan (0.41). A total of 12 categories and 162 alkaloid metabolites were detected in the three regions. Under the screening conditions of multiple difference ≥ 2 or ≤ 0.5, P<0.05, and VIP ≥ 1, there were 53 significantly changed alkaloid differential metabolites in the comparison group between Yinchuan and Guyuan (including 21 up regulated and 32 down regulated), 25 significantly changed alkaloid differential metabolites between Zhongning and Guyuan (including 16 up regulated and 9 down regulated), and 59 different metabolites with significant changes between Yinchuan and Guyuan (including 31 up regulated and 28 down regulated). Five alkaloid metabolites with significant differences were identified through simultaneous comparison among the three production areas (dihydrocaffeioyl putrescine, panthenol mercaptoethylamine, wolfberry D, acetaminophen, and 3,4-dimethoxybenzamide). The KEGG database pathway enrichment analysis found that the five alkaloid metabolites with significant differences were mainly distributed in four metabolic pathways, namely metabolic pathway, biosynthesis of secondary metabolites, carbapenem biosynthesis, and pantothenate and CoA biosynthesis pathways. This study provides a theoretical basis for nutritional exploration of ‘Ningqicai No.1’ goji leaves from different regions in Ningxia.

    参考文献
    [1] 李克剑, 李伊姣, 王储, 等.菜用枸杞叶的营养价值及营养等级评价[J].中国食物与营养, 2016, 22(4): 69-73.LI K J, LI Y J, WANG C, et al.Nutrition value and nutritional rating of vegetable wolfberry leaves[J].Food and Nutrition in China, 2016, 22(4): 69-73.
    [2] 廖家乐, 方甜, 范艳丽.枸杞叶黄酮对胰脂肪酶活性的抑制作用[J].中国食品学报, 2022, 22(5): 43-53.LIAO J L, FANG T, FAN Y L.Inhibitory effects of Lycium barbarum leaves flavonoids on pancreatic lipase activity[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(5): 43-53.
    [3] LEI Z L, CHEN X Q, CAO F L, et al.Phytochemicals and bioactivities of goji (Lycium barbarum L.and Lycium chinense Mill.) leaves and their potential applications in the food industry: A review[J].International Journal of Food Science & Technology, 2021, 57(3): 1451-1461.
    [4] YU C X, CHEN Y H, AHMADI S, et al.Goji berry leaf exerts a comparable effect against colitis and microbiota dysbiosis to its fruit in dextran-sulfate-sodium-treated mice[J].Food & Function, 2023, 14(7): 3026-3037.
    [5] YANG L L, LIANG Q, ZHANG Y J, et al.Variation of phytochemical composition of Lycium chinense leaves as an endemic high-value healthy resource[J].Scientia Horticulturae, 2021, 281(8): 109910.
    [6] 邹立思, 李会伟, 严辉, 等.枸杞叶新资源药材的生药学研究[J].中国现代中药, 2022, 24(1): 20-27. ZOU L S, LI H W, YAN F, et al.Pharmacognostic study of Lycii folium[J].Modern Chinese Medicine, 2022, 24(1): 20-27.
    [7] 祝元春, 钱涛, 周小波, 等.叶用枸杞应用现状及产业发展策略[J].现代园艺, 2022, 45(24): 37-38.ZHU Y C, QIAN T, ZHOU X B, et al.Application status and industrial development strategy of leaf Lycium barbarum[J].Modern horticulture, 2022, 45(24): 37-38.
    [8] CRISTINA A, MEIRA D, JOSE D, et al.Recent trends in pharmacological activity of alkaloids in animal colitis: Potential use for inflammatory bowel disease[J].Evidence-Based Complementray and Alternative Medicine, 2017, 2017: 8528210.
    [9] ARIJIT M, ARIJIT G, CARMELA F, et al.Alkaloids for cancer prevention and therapy: Current progress and future perspectives[J].European Journal of Pharmacology, 2019, 858: 172472.
    [10] 刘建飞, 巩媛, 杨军丽, 等.枸杞属植物中生物碱类成分研究进展[J].科学通报, 2022, 67(4/5): 332-350.LIU J F, GONG Y, YANG J L, et al.Advance on alkaloids of Lycium genus[J].Chinese Science Bulletin, 2022, 67(4/5): 332-350.
    [11] 康子悦, 沈蒙, 葛云飞, 等.基于植物广泛靶向代谢组学技术探究小米粥中酚类化合物组成及其抗氧化性[J].食品科学, 2021, 42(4): 206-214.KANG Z Y, SHEN M, GE Y F, et al.Analysis of phenolic composition in millet porridge using widely-targeted metabolomics and evaluation of antioxidant activity[J].Food Science, 2021, 42(4): 206-214.
    [12] 沈央红, 方金玉, 朱军莉, 等.代谢组学在食品质量安全领域的应用进展[J].食品科学, 2023, 44(5): 282-289.SHEN Y H, FANG J Y, ZHU J L, et al.Advance in the application of metabonomics in the field of food safety and quality[J].Food Science, 2023, 44(5): 282-289.
    [13] 洪雅萍, 谷梦雅, 高婷, 等.福州单瓣茉莉和双瓣茉莉不同组织的广泛靶向代谢组学分析[J].食品科学, 2023, 44(8): 184-193.HONG Y P, GU M Y, GAO T, et al.Widely targeted metabolomic analysis of different tissues of fuzhou single- and double-petal jasmines[J].Food Science, 2023, 44(8): 184-193.
    [14] 潘诗哲, 但凡, 樊洁敏, 等.响应面法优化荷叶生物碱盐提取工艺及其纯化[J].精细化工, 2019, 36(8): 1598-1603, 1609.PAN S Z, DAN F, FAN J M, et al.Optimization of extraction process by response surface methodology and purification of alkaloid salts from lotus leaves[J].Fine Chemicals, 2019, 36(8): 1598-1603, 1609.
    [15] 中华人民共和国农业部.甜菜中甜菜碱含量测定比色法比色法: NY/T 1746-2009[S].北京: 中国标准出版社, 2009: 1-4.Ministry of Agriculture of the People's Republic of China.Colorimetric method for the determination of betaine content in sugar beets colorimetric method: NY/T 1746-2009[S].Beijing: China Standard Press, 2009: 1-4.
    [16] ZHANG Q A, WANG X, SONG Y, et al.Optimization of pyrogallol autoxidation conditions and its application in evaluation of superoxide anion radical scavenging capacity for four antioxidants[J].Journal of AOAC International, 2016, 99(2): 1-8.
    [17] ZHANG S l, DENG P, XU Y C, et al.Quantification and analysis of anthocyanin and flavonoids compositions, and antioxidant activities in onions with three different colors[J].Journal of Integrative Agriculture, 2016, 15(9): 2175-2181.
    [18] VAN DER WERF R, MARCIC C, KHALIL A, et al.ABTS radical scavenging capacity in green and roasted coffee extracts[J].LWT - Food Science and Technology, 2014, 58(1): 77-85.
    [19] 范艳丽, 韩丽娜, 付丽霞, 等.枸杞叶黄酮类化合物体外清除自由基作用研究[J].中国调味品, 2017, 42(12): 32-37.FAN Y L, HAN L N, FU L X, et al.Study on free radical scavenging effect of flavonoids from lycium barbarum leaves in vitro[J].China Condiment, 2017, 42(12): 32-37.
    [20] 龚受基, 李佳银, 杨新河, 等.傅里叶变换红外光谱分析6种不同陈化时间六堡茶[J].食品科学, 2012, 33(24): 222-225.GONG S J, LI J Y, YANG X H, et al.Fourier transform infrared spectroscopic analysis of Liupu tea with different aging times[J].Food Science, 2012, 33(24): 222-225.
    [21] 王志睿, 张兰兰, 黄昌全, 等.粤东产钩吻生物碱提取物的红外光谱与核磁共振谱分析[J].第一军医大学学报, 2005, 25(1): 87-88, 92.WANG Z R, ZHANG L L, HUANG C Q, et al.Infrared spectrum and nuclear magnetic resonance analysis of the alkaloid extract of Gelsemium produced in east Guangdong province[J].Journal of the First Military Medical University, 2005, 25(1): 87-88, 92.
    [22] ZHANG J D, ZHOU Q, YANG G Y, et al.The agronomic traits, alkaloids analysis, FT-IR and 2DCOS-IR Spectroscopy identification of the low-nicotine-content nontransgenic tobacco edited by CRISPR-Cas9[J].Molecules (Basel, Switzerland), 2022, 27(12): 3817-3817.
    [23] 冯玉超, 王长远, 李雪, 等.黑龙江省不同产地水稻的GC-MS代谢物差异分析[J].食品科学, 2019,40(2): 208-214.FENG Y C, WANG L W, LI X, et al.Effect of geographical origin on rice metabolites as analyzed by gas chromatography-mass spectrometry[J].Food Science, 2019, 40(2): 208-214.
    [24] 严淘, 杨敏敏, 施琳, 等.菊花不同提取物代谢组学分析及其抗氧化活性功效物质成分筛选[J].食品工业科技, 2021, 42(16): 8-19.YAN T, YANG M M, SHI L, et al.Metabonomics analysis of different extracts from Chrysanthemum morifolium and screening of its antioxidant active components[J].Science and Technology of Food Industry, 2021, 42(16): 8-19.
    [25] 陈华武.布洛芬混悬液与对乙酰氨基酚在小儿高热治疗中的效果[J].中国实用医药, 2022, 17(9): 133-135.CHEN H W.The effect of ibuprofen suspension and paracetamol in the treatment of children with high fever[J].China Practical Medicine, 2022, 17(9):133-135.
    [26] BERGSTROM A, LIPCSEY M, LARSSON A, et al.Acetaminophen attenuates pulmonary vascular resistance and pulmonary arterial pressure and inhibits cardiovascular collapse in a porcine model of endotoxemia[J].Shock, 2023, 59(3): 442-448.
    [27] 罗新娟, 马蕊, 闫宙洁.布洛芬混悬液联合对乙酰氨基酚治疗小儿高热的临床疗效[J].当代医学,2022, 28(18): 131-133.LUO X J, MA R, YAN Z J.Clinical efficacy of ibuprofen suspension combined with acetaminophen in the treatment of children with hyperthermia[J].Contemporary Medicine, 2022, 28(18): 131-133.
    [28] ZHANG J S, AI Z Y, HU Y, et al.Remarkable impact of commercial sterilizing on ginsenosides transformation in fresh ginseng pulp based on widely targeted metabolomics analysis[J].Food Chemistry, 2022, 15: 100415.
    [29] ZHOU J N, HOU D H, ZOU W Q, et al.Comparison of widely targeted metabolomics and untargeted metabolomics of wild Ophiocordyceps sinensis[J].Molecules, 2022, 27(11): 1-13.
    [30] 张舒,王长远, 冯玉超, 等.气相色谱-质谱联用代谢组学技术分析不同产地稻米代谢物[J].食品科学,2021, 42(8): 206-213.ZHANG S, WANG C Y, FENG Y C, et al.Analysis of metabolites in rice produced in different regions by GC-MS-based metabonomics[J].Food Science, 2021, 42(8): 206-213.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

武彧兴,马瑞雪,卢顺,张惠玲,范艳丽.宁夏不同产地枸杞叶生物碱组成与活性差异[J].中国食品学报,2024,24(10):394-404

复制
分享
文章指标
  • 点击次数:40
  • 下载次数: 42
  • HTML阅读次数: 26
  • 引用次数: 0
历史
  • 收稿日期:2023-10-20
  • 在线发布日期: 2024-12-16
文章二维码
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器,其他浏览器不建议使用!

漂浮通知


×
《中国食品学报》杂志社招聘编辑