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首页 > 过刊浏览>2024年第24卷第11期 >348-358. DOI:10.16429/j.1009-7848.2024.11.032
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基于蛋白组学分析不同产地枸杞黄酮类化合物的差异
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作者单位:

(宁夏大学食品科学与工程学院 银川 750021)

基金项目:

国家自然科学基金项目(32160534)


The Difference of Biosynthesis of Flavonoids in Goji Berry from Different Regions Based on Proteomic Analysis
Author:
Affiliation:

(College of Food Science and Engineering， Ningxia University， Yinchuan 750021)

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    摘要:

    目的:从蛋白质组学的角度解析不同产地“宁杞1号”枸杞黄酮类化合物的差异。方法:以宁夏、青海和甘肃成熟期“宁杞1号”枸杞果实为研究对象，利用串联质谱标签(TMT)结合液相色谱-串联质谱联用(LC-MS/MS)技术，探究不同产地枸杞的蛋白差异。结果:3个比较组共鉴定出716个差异表达蛋白(DEPs)，KEGG通路富集共发现4条与黄酮类化合物相关的代谢途径，其中苯丙烷生物合成途径在3个比较组中均被显著富集(P < 0.05)，表明苯丙烷生物合成途径在枸杞黄酮合成中可能做出较大贡献。筛选出24个与黄酮合成相关的关键差异表达蛋白，发现苯丙氨酸解氨酶(PAL)、肉桂酸4-羟化酶(C4H)、查尔酮异构酶(CHI)、查尔酮合酶(CHS)等可能是宁夏、青海和甘肃枸杞黄酮类化合物生物合成的重要DEPs。结论:从蛋白质层面鉴定出与枸杞黄酮类化合物生物合成相关的差异蛋白，初步推测枸杞黄酮类化合物合成代谢通路，为探究不同产地枸杞黄酮生物合成差异奠定基础。

    Abstract:

    Objective: To analyze the differences of goji flavonoids from different origins from the perspective of proteomics. Methods: The protein differences of goji berries from different origins were explored by tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology in Ningxia， Qinghai and Gansu. Results: A total of 716 differential expressed proteins(DEPs)， and a total of four metabolic pathways associated with flavonoids were found in the KEGG pathway， and the phenylpropane biosynthesis pathway was significantly enriched in all three comparison groups (P < 0.05)， indicating that the phenylpropane biosynthesis pathway might make a greater contribution to the synthesis of goji flavonoids. Further screening of 24 key differentially expressed proteins related to flavonoid synthesis， it was found that phenylalanine ammonia-lyase (PAL)， cinnamic acid 4-hydroxylase (C4H)， chalcone isomerase(CHI)， chalcone synthase (CHS)， etc. might be important DEPs for the biosynthesis of flavonoids in Ningxia， Qinghai and Gansu. Conclusion: The differential proteins related to the biosynthesis of goji flavonoids were identified from the protein level， and the anabolic pathway of goji flavonoids was preliminarily speculated， which laid a foundation for exploring the differences in the biosynthesis of goji flavonoids in different origins.

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孙霞芝,马瑞雪,杨超,范艳丽.基于蛋白组学分析不同产地枸杞黄酮类化合物的差异[J].中国食品学报,2024,24(11):348-358

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  • 收稿日期:2023-11-15
  • 在线发布日期: 2024-12-25
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