降解亚硝酸盐乳酸菌的筛选及其生物特性研究
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(1.安徽科技学院 食品科学与工程学院 安徽滁州 239000;2.安徽省农业科学院农产品加工研究所 合肥 230031;3.安徽省食品微生物发酵与功能应用工程实验室 合肥 230031)

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安徽省科技重大专项(202003a06020007)


Screening and Biological Characteristics of Nitrite-degrading Lactic Acid Bacteria
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(1.College of Food Science and Engineering, Anhui Science and Technology University, Chuzhou 239000, Anhui;2.Institute of Agro-products Processing Research, Anhui Academy of Agricultural Sciences, Hefei 230031;3.Anhui Engineering Laboratory of Food Microbial Fermentation and Functional Application, Hefei 230031)

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    摘要:

    从自然发酵毛竹笋中筛选产酸且降解亚硝酸盐强的乳酸菌,为开发功能性发酵竹笋产品提供优质安全的菌株。乳酸菌经形态学、生理生化、有害代谢产物试验以及16S rDNA分子生物学鉴定和安全性评价,并通过主成分分析法综合性评价菌株的生长能力、产酸能力、降解亚硝酸盐能力、抗氧化活性和耐受性等生物特性,结果表明,11株菌产酸性能好,亚硝酸盐降解率在90%以上。其中,7株菌为γ-溶血的安全菌株,经鉴定,PM4、PM7、PM13为乳脂乳球菌,PM9为乳酸乳球菌乳亚种,PM10为肠膜明串珠菌,PM11、PM17为发酵粘液乳杆菌。7株菌株适应能力强、生长速率快,产酸能力强,且具有较强的抗氧化活性;菌株PM10、PM11耐盐能力最好;菌株PM10、PM11和PM13具有较强的酸耐受性;7株菌均具有较好的亚硝酸盐耐受性。基于主成分分析的生物特性综合评分排名前3名的菌株分别为PM10、PM11和PM13。肠膜明串珠菌PM10、发酵粘液乳杆菌PM11和乳脂乳球菌PM13具有良好的发酵特性和耐受性,可作为功能性微生物资源,上述结果为研发功能性发酵竹笋产品提供理论参考。

    Abstract:

    Lactic acid bacteria with high acid production and strong nitrite degradation ability were screened from natural fermented moso bamboo shoots, as to provide high-quality and safe strains for the development of functional fermented bamboo shoot products. The lactic acid bacteria strains were identified and assessed through morphological, physiological, biochemical, and harmful metabolite tests, along with 16S rDNA molecular identification and safety evaluations. Their biological characteristics-including growth capacity, acid production, nitrite degradation efficiency, antioxidant activity, and tolerance-were comprehensively analyzed using principal component analysis (PCA). The results showed that the 11 strains of bacteria had good acid-producing performance, and the nitrite degradation rate was above 90%. Among them, seven strains were safe strains for γ-hemolysis, and PM4, PM7, and PM13 were identified as Lactococcus cremoris, PM9 was identified as Lactococcus lactis subsp. Lactis, PM10 was identified as Leuconostoc mesenteroides, PM11 and PM17 were identified as Limosilactobacillus fermentum. The seven strains were adaptable, showed faster growth rates, stronger acid production capacity and antioxidant activity. Strains PM10 and PM11 showed better tolerance to sodium chloride. Strains PM10, PM11 and PM13 showed stronger tolerance to acid. All strains showed better tolerance to nitrite. The top three strains ranked in the composite score of biological properties based on PCA were PM10, PM11 and PM13, respectively. In summary, the lactic acid bacteria Leuconostoc mesenteroides PM10, Limosilactobacillus fermentum PM11 and Lactococcus cremoris PM13 had good fermentation characteristics and tolerance. These findings established a theoretical basis for the development of functional fermented bamboo shoot products.

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彭梦灵,朱倩,杨松,谷佳玉,杜京京,伍玉菡,郭家刚,江舰,杨剑婷.降解亚硝酸盐乳酸菌的筛选及其生物特性研究[J].中国食品学报,2025,25(5):134-145

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  • 收稿日期:2024-05-11
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  • 在线发布日期: 2025-06-24
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