Hyperuricemia seriously affects body health， and supplementing lactic acid bacteria (LAB) probiotics has become a new method for treating high uric acid. In the study， LAB with inhibitory activity against nucleosides and xanthine oxidase was screened from traditional pickled vegetables using the high-performance liquid chromatography (HPLC) and uric acid generation method. The probiotic characteristics of LAB were evaluated through acid resistance， bile salt resistance， antioxidant activity， and in vitro digestion stability. Furthermore， the degradation ability of LAB towards guanosine and inosine was determined after simulating artificial intestinal fluid digestion. The results showed that three strains LAB， with high inhibitory activity against nucleoside and xanthine oxidase (XOD)， were screened from 27 strains LAB， namely JYC-4， JYC-6， and JYC-7. Among them， strain JYC-7 exhibited higher degradation rate to inosine and guanosine at 99.64% and 99.80%， respectively. Its cell metabolite and content showed inhibition rates against XOD at 73.91% and 68.46%， respectively. The three strains LAB were identified as Lactiplantibacillus plantarum. In addition， the three L. plantarum exhibited good acid resistance， bile salt resistance， and antioxidant activity. After simulating artificial gastrointestinal digestion， the viable count of L. plantarum ranged from 2.8×106-5.7×106 CFU/mL， and the degradation rates of guanosine and inosine were 90.77%-99.10% and 81.97%-93.49%， respectively. The L. plantarum exhibited excellent probiotic properties， providing new microbial agents for prevention and treatment of hyperuricemia.