提取方法对无花果果胶结构和理化特性的影响
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(中国农业科学院农产品加工研究所 农业农村部农产品加工与贮藏重点实验室 北京 100193)

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贵州省科技计划项目(黔科合中引地(2024)025,黔科合平台[2024]037);石家庄市农业科技项目(24039)


Effects of Extraction Methods on Structure and Physicochemical Properties of Ficus carica Linn Pectin
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(Key Laboratory of Agricultural Product Processing and Storage, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193)

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    摘要:

    为探讨不同提取方式对无花果果胶结构特征、理化特性以及功能特性的影响,采用酸提、碱提、酶提和水提4种方法提取无花果果胶,并表征制备的果胶单糖组成、分子质量及酯化度等结构特征。结果表明,4种提取方法的果胶得率在3.65%~6.19%范围,其中,碱提方法的果胶得率(6.19%)最高,酶提方法的果胶得率(3.65%)最低;无花果果胶半乳糖醛酸含量为91.02~248.09 mg/g,另外,阿拉伯糖、半乳糖、鼠李糖等中性糖含量也较为丰富;酸提方法的果胶分子质量较大(2.00×104~1.59×105 u),表明果胶分子更为完整;不同提取方式制备果胶的酯化度为3.92%~44.42%,均为低酯果胶(LMP),其中,酸提果胶的酯化度最高(44.42%),碱提果胶的酯化度最低(3.92%)。无花果果胶具备良好的剪切稀释特性(105~103 MPa)、热稳定性(降解温度约150 ℃)以及抗氧化能力,可显著抑制α-淀粉酶和α-葡萄糖苷酶活性,其中酸提果胶的抑制活性更强。本研究结果可为无花果果胶提取和利用提供科学依据。

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    This article aimed to explore the effects of different extraction methods on the structural, physicochemical and functional characteristics of Ficus carica Linn pectin. The pectin from figs was extracted using four methods: Acid extraction, alkali extraction, enzymatic extraction, and water extraction. The monosaccharide composition, molecular weight (Mw) and esterification degree (DE) of pectin prepared by different extraction methods were characterized. The results showed that the yields the four kinds of pectin were 3.65%-6.19%, among which the yield of alkali extraction was the highest and the enzyme extraction was the lowest. The galacturonic acid contents of the four kinds of pectin ranged 91.02-248.09 mg/g, other neutral sugars such as acarbose, galactose and rhamnose contents were also relatively rich. The molecular weight of acid extraction was larger (2.00×104-1.59×105 u), indicating that the extracted pectin molecule was more complete. The degree of esterification (DE) were 3.92%-44.42%, all four kinds of pectin were low methoxyl pectin (LMP), among them, DE of acid extraction was the highest, and that of alkali extraction was the lowest. Fig pectin was confirmed to be a natural LMP (DE: 3.92%-44.42%), exhibiting excellent shear-thinning behavior (105-103 MPa), thermal stability (degradation temperature around 150 ℃), and antioxidant capacity. It significantly inhibits α-amylase and α-glucosidase activities, with acid-extracted pectin demonstrating stronger inhibitory effects. These results provided a basis for the extraction and utilization of Ficus carica Linn pectin.

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马科科,毕金峰,易建勇.提取方法对无花果果胶结构和理化特性的影响[J].中国食品学报,2025,25(5):290-302

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  • 收稿日期:2024-05-20
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  • 在线发布日期: 2025-06-24
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