高粱阿拉伯木聚糖的分离提取及其特性
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(1.浙江万里学院 生物与环境学院 浙江宁波 315100 ;2.中国农业大学食品科学与营养工程学院 北京 100083)

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浙江省“生物工程”一流学科课题项目(ZS2022010);浙江省重点研发计划项目(2020C02042);山东省重点研发计划项目(2022CXGC010602);宁波市公益类科技计划项目(2022S149);宁波市自然科学基金项目(2023J295)


Isolation, Extraction and Characteristic of Sorghum Bran ArabiNOxylans
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(1.College of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315100, Zhejiang;2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083)

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    摘要:

    以高粱麸皮为原料,分离提取高粱阿拉伯木聚糖(SAX),探究碱液浓度、提取时间和温度等关键因素对SAX得率及阿魏酸含量的影响,确定最佳提取工艺。分析由不同来源高粱麸皮得到的SAX的基本成分、单糖组成、分子质量及分子结构差异,并对其流变性、凝胶性、乳化性及抗氧化能力等进行评价。结果表明,SAX最佳提取条件为NaOH浓度0.25 mol/L,提取时间4 h,温度25 ℃,在此条件下其得率和阿魏酸含量均较高,分别为4.63%和11.79 μg/10 mg。不同来源SAX分子组成及理化特性均有较大差异。3种高粱SAX中,山东红粳高粱SAX具有较高的阿拉伯糖含量(29.60%)和木糖含量(29.30%),较高的分子质量(209.79 ku)和较低的分支度(2.06/1.08)。相比于几种常见食品多糖,山东红粳高粱SAX因富含大量的酚酸和花青素,具有较高的抗氧化活性。与其它2种SAX相比,山东红粳高粱AX具有良好的乳化稳定性和较强的酶促凝胶能力,更有利于后续食品加工及利用。本研究为了解高粱SAX结构组成和功能特性提供了参考,同时为高粱加工副产物的增值利用提供了新途径。

    Abstract:

    Sorghum bran arabiNOxylan (SAX) was isolated from sorghum bran, and the effects of key extraction factors, such as alkali concentration, extraction time, and temperature, on the yield and ferulic acid content of SAX were investigated to determine the optimal extraction process. Additionally, the variations in components, monosaccharide composition, molecular weight, and structure of SAX derived from different sorghum sources were studied. Their rheological properties, gelling ability, emulsification capacity, and antioxidant capabilities were also assessed and analyzed. The results revealed that the optimal conditions for SAX extraction were a 0.25 mol/L NaOH concentration, a 4 h extraction time, and a temperature of 25 ℃. Under these conditions, the yield and ferulic acid content of were relatively high, at 4.63% and 11.79 μg/10 mg, respectively. SAX obtained from different sources exhibited significant differences in molecular composition and physicochemical properties. Among these variants, SAX isolated from Shandong red non-waxy sorghum (SAX-SD) exhibited higher levels of arabinose (29.60%) and xylose (29.30%), a greater molecular weight (209.79 ku), and a lower branching degree(2.06/1.08). Compared to several common food polysaccharides, SAX-SD demonstrated superior antioxidant activity due to its high content of phenolic acids and anthocyanins. Compared with the SAX of the other two sorghum varieties, SAX-SD had good emulsifying stability and strong gelling ability, which was more conducive to subsequent food processing and utilization. This study provides valuable insights into the structural composition and functional properties of SAX while also offering a new avenue for the value-added utilization of sorghum processing by-products.

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颜金鑫,戚向阳,杨华,曹少谦,贾鑫,殷丽君.高粱阿拉伯木聚糖的分离提取及其特性[J].中国食品学报,2025,25(5):303-316

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  • 收稿日期:2024-05-14
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  • 在线发布日期: 2025-06-24
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