To investigate the effect of buckwheat hull flavonoids (BHFs) on apoptosis and migration of human hepatocellular carcinoma HepG2 cells. The inhibitory effect of BHFs on human lung cancer A549 cells， human cervical cancer Hela cells， human hepatocellular carcinoma HepG2 cells and human normal liver L-02 cells was evaluated by MTT colorimetric method， and HepG2 cells were further selected as experimental subjects. Cell morphology was analyzed by Hoechst 33345/PI double staining， JC-1 fluorescence probe detection， Annexin V-FITC/PI flow cytometry， wound healing assay， and Western blot analysis of BHFs pretreatment to investigate the effects of apoptosis and migration of HepG2 cells and the potential mechanism. The results showed that BHFs could effectively inhibit the viability of A549， Hela and HepG2 cells， the inhibitory effect on HepG2 cells was the most significant， the survival rate of HepG2 cells was 53.62% after treatment with 50 μg/mL for 48 h，and had no effect on L-02 cells viability. Compared with the untreated group， BHFs inhibited the proliferation of HepG2 cells in a dose-dependent manner， decreased mitochondrial membrane potential， down-regulated the expression of apoptotic proteins Bcl-2 and Bcl-XL， increased the expression of FADD and cleaved-Caspase-3， and decreased the expression of migratory protein MMP 9， and the effect was significantly superior to that of positive control of rutin. The results indicate that BHFs have anti-tumor ability， and the inhibition of HepG2 cell proliferation may be related to the induction of mitochondrial， death receptor cell apoptosis and the inhibition of cell migration.