The objective of this study was to investigate the potential role of oyster peptide in alleviating alcoholic liver injury and reducing physical fatigue. The molecular weight and amino acid content were determined by liquid chromatography， respectively， and the alleviation effect of oyster peptide on mice alcoholic liver injury and physical fatigue was studied in vivo， set up three doses: low dose group (0.25 g/kg bw)， medium dose group (0.5 g/kg bw) and high dose group (1.0 g/kg bw)， and the corresponding control group. The results showed that the molecular weight of oyster peptide was 85% less than 1 500 u. The total amount of amino acid is 58.44 g/100 g. The in vivo findings demonstrated that the levels of MDA and TG in the livers of mice fed with three different doses of oyster peptide were significantly decreased compared to the model group. Additionally， the levels of GSH were significantly increased compared to the model group. The results of pathological sections of liver tissues suggested that， the pathological liver damage of the high-dose group was alleviated in varying degrees. In the experiments of relieving mice physical fatigue， the swimming time of the middle and high dose groups was significantly increased， and the contents of blood lactic acid and serum urea of the three dose groups were significantly decreased compared with the control group. Collectively， the oyster peptide have small molecular weights and high content of amino， which also have effects on relieving alcoholic liver injury and physical fatigue.