Hemp seeds are rich in functional substances such as proteins， fatty acids and their esters， flavonoids， lignanamides， alkaloids， steroids， and terpenoids. Through the extraction methods of water extraction and alcohol extraction， the components of water and alcohol extracts were analyzed to study the effects of water-soluble and alcohol soluble substances on mice intestinal lubrication， defecation， and antioxidant properties， providing a basis for the functional development and application of hemp seed. The component analysis results showed that the water extract was rich in protein and total sugar， while the alcohol extract was significantly characterized by high levels of fat， cannabinoid (CBD)， and total phenols. Different extraction methods resulted in significant differences in the composition of hemp seed extract， which were further validated through in vivo animal experiments， the mice were divided into a solvent control group (BL)， a model control group (M)， a low-dose group of hemp seed water extract (MS-L)， a high-dose group of hemp seed water extract (MS-H)， a low-dose group of Hemp seed alcohol extract (MC-L)， and a high-dose group of hemp seed alcohol extract (MC-H). The defecation test lasted for 7 days， and the antioxidant test lasted for 30 days. The defecation test was conducted to test the small intestine movement and defecation of mice， while the antioxidant test was conducted to measure the levels of antioxidant enzymes such as SOD， MDA， GSH， PCO in the serum. The results indicated that， compared with the solvent control group， the constipation model and ethanol-induced oxidative stress model were successfully established. Compared with the M group， MC-H group showed significant differences in various indicators in the defecation experiment. Overall， both the alcohol extract and water extract were able to improve small intestine movement and defecation in constipated mice， and alcohol extract of hemp seed had a better effect on the defecation in mice. In the antioxidant experiment， the PCO and MDA contents of the ethanol and water extract were significantly reduced， while the SOD and GSH contents were significantly increased， indicated strong antioxidant capacity. Therefore， hemp seed can effectively improve constipation in mice and has strong antioxidant capacity.