To study the inhibitory effects and mechanisms of quercetin and anthocyanin alone， as well as the combined inhibitory effects of the two on digestive enzymes， providing new theoretical basis for the interaction between phenolic substances and digestive enzymes， and to explore the degree of regulating blood glucose homeostaction in C57BL/6J obese mice. The inhibitory mechanisms of quercetin and anthocyanin on α-amylase and α-glucosidase activity were studied by ultraviolet spectroscopy， fluorescence spectroscopy， enzyme inhibition kinetics double reciprocal method， and molecular simulation docking， and an obese mouse model was established to measure relevant blood serum indicators to explore its in vivo regulation effects. The inhibitory effects of the inhibitors showed a dose-dependent relationship， and the IC50 values of quercetin and anthocyanin for α-amylase were 0.34 mg/mL and 0.027 mg/mL， respectively. The IC50 values of quercetin and anthocyanin for α-glucosidase were 0.30 mg/mL and 0.01 mg/mL， respectively. According to the IC50 values， set the compound ratio to 340∶27 to inhibit α-amylase， and 30∶1 to inhibit α-glucosidase， the CI values for α-amylase and α-glucosidase were set to 0.047 and 0.20， respectively， with synergistic inhibitory effects. The results of the mouse experiment showed that anthocyanin and quercetin could effectively improve the disordered state of blood glucose in obese mice. This study provides a certain basis for the development and utilization of foods rich in anthocyanin and quercetin， and is conducive to promoting the regulation of postprandial blood glucose by inhibitors.