For identifying the content of purine in different parts of sea fish. In this paper， a high-performance liquid chromatogram （HCLP） method was developed in order to determinate the contents of guanine， hypoxanthine， xanthine and adenine in edible parts and internal organs of seawater fish. Purine bases were separated using Agilent ZORBAX Eclipse XDB-C18 （4.6 mm × 250 mm， 5 μm） chromatographic column with water-methanol-ice acetic acid-20% tetrabutyl ammonium hydroxide（V/V/V/V = 879/100/15/6） as mobile phase， the flow rate was set at 0.8 mL/min， column temperature was 30 ℃， and wavelength of UV detection was at 254 nm. The sample volume was 10 μL. Four kinds of purines had a good linear relation between the concentration and peak area in the range of 0.1-400 mg/L. The correlation coefficient was 0.9998-1.0000， the limit of detection （LOD） was between 0.0465-0.1056 mg/L， method precision of RSD% was between 0.0200%-0.7000%. The repeatability of sample treatment adenine， guanine， hypoxanthine， xanthine is 1.2421%， 0.9711%， 0.8836%， 1.9727% respectively. The recovery rate was between 94.2888%-102.9188%. So the method was suitable for the determination of four purines in marine fish. The test results showed that the total purine contents of edible part （eye， belly of fish， the back of the fish， fish skin） in sea bass were the highest， which was followed by turbot， golden hamster， yellow croaker， sciaenops ocellatus， Pedal fish and gadus in oeder.