文章摘要
呼鑫荣;饶欢;熊海宽;薛文通.松露的体外抗氧化性评价及对秀丽线虫的体内抗氧化[J].中国食品学报,2020,20(7):27-33
松露的体外抗氧化性评价及对秀丽线虫的体内抗氧化
Evaluation of Truffle’s Antioxidant Activity in Vitro and Its Antioxidative Effect on C. elegans
  
DOI:
中文关键词: 松露  抗氧化  秀丽线虫
英文关键词: truffle  antioxidant capacity  C. elegans
基金项目:国家重点研发计划项目(2019YFC1605000)
作者单位
呼鑫荣;饶欢;熊海宽;薛文通 北京食品营养与人类健康高精尖创新中心中国农业大学食品科学与营养工程学院永仁野森达菌业有限公司 
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中文摘要:
      对松露的自由基清除率、铁离子螯合能力、总还原能力进行检测,评价松露不同极性提取物的体外抗氧化能力,筛选出抗氧化能力最好的提取物,并对其进行秀丽线虫体内抗氧化测试。结果表明:松露不同极性提取物抗氧化能力存在显著差异,综合抗氧化能力排序为:水提物>甲醇提取物>乙酸乙酯提取物>石油醚提取物。松露提取物可有效延长线虫寿命,增加线虫产卵量以及提高线虫急性应激能力。50 μg/mL的松露水提物处理可将线虫的平均寿命延长31.86%,产卵量增加36.55%,并可将急性热应激和急性氧化应激环境下线虫的寿命分别延长28.70%,59.52%。本研究结果为松露的精深加工利用提供了理论支撑。
英文摘要:
      The free radical scavenging rate, iron ion chelating ability and total reducing capacity of truffles were tested to evaluate the antioxidant capacity of truffle extracts, and the extracts with the best antioxidant capacity were screened out. And then explore the effects of truffle on C. elegans. The results showed that there were significant differences in the antioxidant capacity of different polar extracts of truffle, and the comprehensive antioxidant capacity was ranked as follows: water extract > methanol extract > ethyl acetate extract > petroleum ether extract. The treatment with 50 μg/mL truffle water extract could increase the average lifespan of nematodes by 31.86% and fecundity by 36.55%, and prolong the lifespan of C. elegans in acute heat stress and acute oxidative stress environments by 28.70% and 59.52% respectively. This study provided ideas and theoretical support for the use of truffles for fine and deep processing.
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