乳清蛋白水解物对嗜酸乳杆菌的益生作用
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The Beneficial Effect of Whey Protein Hydrolysate on Lactobacillus acidophilus JC1
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    摘要:

    益生菌具有多种生理功能,尤其在维护人体肠道健康等方面具有重要作用。然而,其也存在易受外界环境和胃环境影响而死亡的局限性。寻找能促进益生菌增殖和耐受性且对人体无害的新物质成为研究热点。本文以嗜酸乳杆菌为研究对象,探究乳清蛋白水解物对嗜酸乳杆菌的益生作用。将乳清蛋白水解物添加到嗜酸乳杆菌的液体培养基中,定期测定活菌数的变化,并用细菌生长动力学模型描述细菌的生长情况。此外,测定嗜酸乳杆菌对乳清蛋白水解物的利用情况,探究其对嗜酸乳杆菌生物被膜和胞外聚合物(EPS)的影响。结果表明:随乳清蛋白水解物添加量的增加,对嗜酸乳杆菌的生长促进作用也增强;细菌的延滞期缩短并提前进入对数期。添加乳清蛋白水解物后细菌生物被膜含量和胞外聚合物含量显著增加。乳清蛋白水解物的益生作用很可能是其增强了嗜酸乳杆菌的群体感应效应,从而促进细菌抵御外界不良环境影响的能力并促进细菌的生长繁殖。

    Abstract:

    Probiotics have many physiological functions, especially in the maintenance of human intestinal health. However, it also has the limitation of being vulnerable to the influence of external environment and gastric environment. Therefore, it has become a research hotspot to find new substances which are harmless to human body and can promote the proliferation and tolerance of probiotics. Therefore, Lactobacillus acidophilus was taken as the research object to explore the beneficial effect of whey protein hydrolysate on Lactobacillus acidophilus JC1. The whey protein hydrolysate was added to the liquid medium of L. acidophilus JC1, and the change of the number of living bacteria was measured regularly, and the bacterial growth was described by the kinetic equation of microbial growth. In addition, the utilization of L. acidophilus JC1 to whey protein hydrolysate was determined, and the effects of WPH on biofilm and extracellular polymeric substances(EPS) were explored. The results showed that with the increase of the amount of whey protein hydrolysate, the growth promoting effect was strengthened, and the lag phase of bacteria was shortened and the logarithmic period was entered ahead of time. The content of bacterial biofilm and EPS increased significantly with the addition of whey protein hydrolysate. The beneficial effect of whey protein hydrolysate was likely to be that it enhanced the quorum sensing system of L. acidophilus JC1, thus promoting the ability of bacteria to resist the adverse environmental impact and promoting the growth and reproduction of bacteria.

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丁婷;李勇.乳清蛋白水解物对嗜酸乳杆菌的益生作用[J].中国食品学报,2021,21(6):54-61

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  • 在线发布日期: 2021-07-16
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