基于DNA条形码和矿物元素指纹的三文鱼物种鉴定和产地溯源技术
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浙江省重点研发项目(2018C02041)


Species Identification and Origin Traceability of Salmon Based on DNA Barcode and Mineral Element Fingerprint
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    摘要:

    本研究评估DNA条形码技术在三文鱼真伪鉴别中的适用性,并探讨矿物元素指纹对进口三文鱼产地溯源的可行性。通过PCR特异性扩增测序结合GenBank已有序列,对8属29种鲑科鱼类线粒体COI序列进行遗传距离和系统进化分析。结果显示,鲑科鱼类种间平均遗传距离为0.150,是种内平均遗传距离(0.001)的150倍。在分子系统进化树上,同一鲑科鱼种均聚集于同一独立分支内,且均获得较高的支持率。上述结果表明:基于COI基因的DNA条形码技术可有效应用于三文鱼的真伪鉴别。通过对挪威和智利三文鱼矿物元素指纹数据进行方差分析、主成分分析与判别分析,发现Fe、Zn、Al、Ni、As、Cr、V、Se、Ca、Na等10种矿物元素可作为三文鱼的特征元素表征其地理来源。基于这些元素所建立的判别模型对三文鱼的整体及交叉验证判别率均高达98.8%。结论:矿物元素指纹结合化学计量方法可作为挪威和智利三文鱼产地判别的有效手段。本研究结果为建立和完善我国进口水产品质量安全识别体系提供了技术参考。

    Abstract:

    In this study, we evaluated the applicability of DNA barcode technology in the authentication of salmon and explored the feasibility of mineral element fingerprint for traceability of imported salmon. The mitochondrial cytochrome c oxidase subunit 1 (COI) gene sequences of 29 species belonging to 8 genera in Salmonidae which amplified using a polymerase chain reaction technique or screened from GenBank were used for genetic distance and phylogenetic analysis. The results showed that the average interspecific genetic distance is 0.150, which is 150 times higher than the average intraspecific genetic distance (0.001). The phylogenetic analysis showed that the COI haplotypes of each species were clustered in a respective branch in the neighbor-joining tree with high support rates. The above results highlighted that the DNA barcode technology based on COI gene can effectively apply in the authentication of salmon. In addition, the mineral element fingerprint data of salmon from Norway and Chile were analyzed by the methods of variance comparison, principal component analysis and discriminant analysis. As a result, 10 mineral elements including Fe, Zn, Al, Ni, As, Cr, V, Se, Ca and Na were selected to characterize the geographic origin of salmon. The overall and cross-validation discrimination rates derived from the discriminant model that established by these 10 elements were both 98.8%. Hence, the mineral element fingerprint combined with appropriate stoichiometric method was effective in geographical tracing of Norwegian and Chilean salmon. Overall, our results provide technical references for the establishment and improvement of the quality and safety identification system of imported aquatic products in China.

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王紫晔;王正亮;黄珏;俞晓平.基于DNA条形码和矿物元素指纹的三文鱼物种鉴定和产地溯源技术[J].中国食品学报,2021,21(6):251-258

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  • 在线发布日期: 2021-07-16
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