In order to excavate the exploitation utilization value of Brassica rapa L. ssp. rapa resources， this study analyzed the nutrient composition and amino acid composition of 50 Brassica rapa L. ssp. rapa resources by using atomic absorption spectrophotometry and automatic amino acid analyzer. The results showed that the content of mineral elements of 50 Brassica rapa L. ssp. rapa resources were different， which calcium （Ca）， maghesium （Mg） and potassium （K） were the main element. The total mineral element content of G2 and T11 resources was the highest. Analysis of amino acids showed that G4 recourse had the highest content of total amino acids， essential amino acids in children， essential amino acids， non-essential amino acids， flavor amino acids， sweet amino acids， medicinal amino acids and sulfur-containing amino acids， and 1408 had the highest content of aromatic amino acid （1.09 g/100 g DW）. Amino acid content analysis of Brassica rapa L. ssp. rapa resources showed that the average content of glutamate was the highest （3.35 g/100 g DW） and the content of cystine was the least（0.01 g/100 g DW）. The principal components analysis showed that the five principal components contained most of the information of all tested traits （81.807%）. Clustering analysis revealed that 50 species of turnip resources could be classified into 4 groups at 5 genetic clusters： Group 1 possessed the lowest selenium （Se） content （0.058 mg/kg） and the lowest average amino acid content （9.72 g/100 g DW）. Group 2 possessed the lowest average iron （Fe） content （47.08 mg/kg） and the highest average methionine content. Group 3 possessed the middle and lower levels of mineral elements and amino acids. Group 4 possessed the highest average of mineral elements and amino acids indicating that the better exploitation utilization value. Comprehensive analysis of all detected components， four excellent Brassica rapa L. ssp. rapa germplasm resources （G2， G4， T11 and 1408） were selected for further study.