In this study,microfibril film was prepared by konjac glucomannan (KGM) combined with polyacrylamide (PAAM) via microfluidic spinning technology.The rheological properties of the obtained KGM/PAAM sol were measured,the microfibril film was characterized by FT-IR,GTA,SEM and XRD.The ofloxacin particles were further loaded into the microfiber for antibacterial experiments.The results revealed that:(1) The optimum ratio of KGM/PAAM sol was:KGM:PAAM (mass ratio) = 1:1;(2) The KGM/PAAM microfibril film prepared formed an ordered network structure,with the fiber diameter of 100 μm,and the thermal stability of the film was enhanced.(3) The antibacterial experiment results reveled that KGM/PAAM microfibril film had good antibacterial properties,it had obvious antibacterial effect on Vibrio parahaemolyticus,and the inhibition zone diameters for Escherichia coli and Staphylococcus aureus were 39.6 mm and 31.5 mm,respectively.The purpose of this work is to provide a theoretical basis for the development of new antibacterial materials.