Objective: The serotype and genotype of 399 Salmonella isolates recovered from retail raw chickens collected from Guangdong province (n=130)， Guangxi province (105)， Fujian province (82) and Shanghai City (82) were studied to protect food safety. Methods: The serotype was tested and determined using antiserum via serum agglutination method that recommended by the World Health Organization(WHO). The genotype was determined according to the protocol of pulse field gel electrophoresis(PFGE) that issued by the U.S. Centers for Disease Control and Prevention(CDC)， the DNA profiles were clustered using BioNumerics software. Results: Forty-seven serotypes were detected among 399 Salmonella isolates， among which， Salmonella Enteritidis (17.04%)， S. Typhimurium (16.04%)， S. Indiana(14.04%)， S. Thompson (9.02%)， S. Agona(7.52%) and S. Rissen (3.76%) were the predominant serotypes. Salmonella Derby， S. Infantis， S. Duesseldorf， S. Edinburgh and S. Bellerue were rarely detected. Salmonella Typhimurium was the most prevalent serotype in Guangdong province (20.00%)； whereas， Salmonella Enteritidis was the common serotype in Guangxi (18.10%) and Fujian province (29.27%)； S. Indiana was the main serotype of Shanghai. PFGE genotype of the isolates with same serotype that recovered from different samples were similar or highly similar within each province. Isolates with same serotype recovered from different samples and provinces exhibited diverse genotypes. Conclusions: Salmonella isolates recovered retail raw chickens collected from Guangdong， Guangxi， Fujian and Shanghai not only exhibited diverse serotypes and genotypes， but also high homology in the same region.