23个柑橘品种果实油胞层类黄酮组分鉴定与抗氧化活性研究
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(浙江大学果实品质生物学实验室/农业农村部园艺植物生长发育与品质调控重点开放实验室/园艺产品冷链物流工艺与装备国家地方联合工程实验室/园艺植物整合生物学研究与应用浙江省重点实验室 杭州 310058)

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国家重点研发计划政府间国际科技创新合作专项(2017YFE0122300);国家自然科学基金项目(32072132,3210932);农业农村部科研杰出人才及其创新团队(果实营养与人类健康创新团队)项目;中央高校基本科研业务费专项基金(2020XZZX003-03)


Identification of Flavonoids from the Flavedo of 23 Citrus Cultivars Fruit and Their Antioxidant Activity
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(Laboratory of Fruit Quality Biology, Zhejiang University/ Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture and Rural Affairs/ Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory/ Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou 310058)

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    摘要:

    选取23个柑橘品种果实油胞层,采用高分辨质谱等技术鉴定其主要类黄酮组分,研究其抗氧化活性。结果表明:柑橘果实油胞层的总酚含量在4.38~21.10 mg GAE/g DW之间。柑橘果实油胞层综合抗氧化能力最强,最弱的品种分别为“本地早”“爱媛38”。从23种柑橘果实油胞层中共鉴定出53种类黄酮,包括5种黄酮-C-糖苷,4种黄酮-O-糖苷,12种黄烷酮-O-糖苷,32种多甲氧基黄酮(PMFs),有2种类黄酮为首次在柑橘中被鉴定出,分别是根皮素-3',5'-二-C-葡萄糖苷和槲皮-3-O-鼠李糖基己糖苷。油胞层中的黄烷酮类化合物主要为圣草次苷、新圣草次苷、柚皮芸香苷、橙皮苷和香蜂草苷,PMFs主要为川陈皮素、橘皮素、5HPMF和甜橙黄酮。本文进一步挖掘并补充不同柑橘品种组分和抗氧化活性信息,为活性研究提供理论依据,也为消费提供科学指导。

    Abstract:

    In this study, the flavedo of 23 Citrus varieties were selected as materials, and the components and antioxidant capacity of flavonoids were investigated by Liquid Chromatography-Mass spectrometry(LC-MS). The results showed that, the total phenolic content of the flavedo varied from 4.38 mg GAE/g DW to 21.10 mg GAE/g DW; and the varieties with the strongest and weakest comprehensive antioxidant capacity in the flavedo were 'local early' and 'Aiyuan 38'. Using the LC-MS to identify the flavonoids in the flavedo of 23 Citrus varieties, we identified a total of 53 flavonoids, including 5 flavonoid-C-glycoside, 4 flavonoid-O-glycoside, 12 flavanone-O-glycoside, 32 polymethoxyflavone (PMFs). Compared with the existing literature, two flavonoids, phloretin-3',5'-di-C-glucosid and hesperitin-7-O-rhamonoside, were reported for the first time in Citrus. The main flavanones contained in the flavedo are eriocitrin, neoeriocitrin, narirutin, hesperidin, and didymin. The main PMFs in the flavedo are nobiletin, tangeretin, 5HPMF and sinensetin. In conclusion, this article further supplemented information on the components and antioxidant activities of different Citrus varieties, which provided scientific guidance for Citrus consumption and set a theoretical basis for bioactivity studies.

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刘阳,臧文静,梁潇,王岳,李鲜,孙崇德.23个柑橘品种果实油胞层类黄酮组分鉴定与抗氧化活性研究[J].中国食品学报,2022,22(12):234-246

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  • 收稿日期:2021-12-13
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  • 在线发布日期: 2023-01-09
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