To investigate the effect of high CO₂ stress on cell wall metabolism of Actinidia arguta. Based on the ‘Longcheng NO. 2’ as the experimental material， the air-regulated element A (CK2) and air-regulated element B (TR) are respectively pasted， and the air-regulated element CK1 is not pasted， research during the high concentration of CO₂ stress on frozen fruit peel strength， cell wall polysaccharides， cell wall degradation enzymes and reactive oxygen species (ROS)， the microstructure of fruit peel in different treatment groups was analyzed on day 60. The results showed that the suitable storage environment (O₂ content 17.00%-20.92%， CO₂ content 1.63%-2.78%) was formed in CK2 group during cold storage. TR group formed a high concentration of CO₂ stress storage environment (O₂ content is 11.22%-15.55%， CO₂ content is 9.00%-11.93%). Compared with CK1 and CK2， TR group could inhibit the decrease of peel strength， affect the normal decrease of propectin， cellulose and hemicellulose contents， inhibit the increase of water-soluble pectin， and decrease the activities of polygalacturonase (PG)， cellulase (CX)， β -galactosidase (β-Gal) and amylase. The activity of superoxide anion (O₂·-)， content of hydrogen peroxide (H2O₂)， malondialdehyde (MDA) and relative electrical conductivity were increased. At the same time， the structure of pericarp cells was seriously damaged， the epidermis was rough and disorderly， the cell gap was enlarged， and the tissue contour was blurred and disordered. Further analysis showed that the CO₂ content in the TR group exceeded the tolerance threshold during storage， which resulted in the slow decline of fruit rigidity and hardness， affected the process of post-ripening， inhibited the metabolic transformation of cell wall polysaccharide， the accumulation of free radicals in the tissue membrane lipid peroxidation， damaged cell structure， and lost commercial property.