四川腊肉中酵母菌多样性及其特性研究
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(1.四川农业大学食品学院 四川雅安 625014;2.宜宾学院固态发酵资源与利用四川省重点实验室 四川宜宾 644000)

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成都市重大科技应用示范项目(2019YF0900050SN)


Studies on the Diversity and Technological Properties of Yeast Isolated from Sichuan Bacon
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(1.College of Food Science, Sichuan Agricultural University, Ya'an 625014, Sichuan;2.Key Laboratory of Sichuan Province for Solid Fermentation Resources and Utilization, Yibin University,Yibin 644000, Sichuan)

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    摘要:

    从四川不同区域采集39份农家腊肉,测定其微生物类群数量和pH、aw值。分离纯化酵母菌菌株,采用形态、生化特征结合26S rDNA D1/D2区序列进行鉴定,并对其中部分酵母菌的生物学和产香特性进行分析。结果显示,除少数样品外,四川腊肉aw 0.8~0.9,pH 5.0~6.0,乳酸菌和葡萄球菌是其优势菌群,山地丘陵区样品酵母菌数量均超过4 lg (CFU/g)。从四川腊肉中共分离获得113株酵母菌,主要包括季也蒙毕赤酵母、汉逊德巴利酵母、胶红酵母、诞沫假丝酵母、浅白隐球酵母和Naganishia diffluens,其中季也蒙毕赤酵母和汉逊德巴利酵母是优势菌种,分别占总数的28.3%和23.9%。大多数试验菌株具有对低温、高盐、偏酸环境的良好耐受性,广泛的胞外脂肪酶和过氧化氢酶活性,接种模拟肉汤发酵后可显著提高3-甲基丁醇和苯乙醇的含量,减少糠醛、苯甲醛和二甲基苯甲醛的生成。四川腊肉酵母菌具有丰富的多样性和良好的应用特性,以及较好的开发前景。

    Abstract:

    The number of microbial groups, pH and aw values of 39 bacons collected from different regions of Sichuan were determined. The yeast strains were isolated and purified from the PDA counting plate, identified by morphological and biochemical characteristics combined with 26S rDNA D1/D2 region sequence, and evaluated biological and aroma-producing characteristics of 24 strains of yeast. The results showed that the range of aw and pH of Sichuan bacon were 0.8-0.9 and 5.0-6.0 respectively except for a few samples. Lactic acid bacteria and Staphylococci are the dominant microorganisms in Sichuan bacon, and the number of yeasts in samples collected from mountainous areas all exceeded 4 lg (CFU/g). A total of 113 yeast strains were isolated from Sichuan bacon, including Meyerozyma guilliermondii, Debaryomyces hansenii, Rhodotorula mucilaginosa, Candida zeylanoides, Cryptococcus albidus and Naganishia diffluens, of which M. guilliermondii and D. hansenii were found as the dominant species accounted for 28.3% and 23.9%, respectively. Most of the tested strains exhibited good tolerance to processing environment and extracellular lipase and catalase activity. Interestingly, it can significantly increased the content of 3-methylbutanol and phenethyl alcohol and reduced the production of furfural, benzaldehyde and dimethyl benzaldehyde in the three inoculated groups. In conclusion, yeast of Sichuan bacon has rich diversity and good technological properties, and has potential application value and development prospects.

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王松,唐林,郭柯宇,刘书亮,杨勇.四川腊肉中酵母菌多样性及其特性研究[J].中国食品学报,2023,23(4):334-344

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  • 收稿日期:2022-04-18
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  • 在线发布日期: 2023-05-26
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