In order to investigate the effect of complex probiotics on immune function of cyclophosphamide-induced immunosuppression model mice， BALB/c mice were randomly divided into blank group and model group (perfused with normal saline)， positive control group [perfused with levamisole hydrochloride (LEV， 10 mg/kg)]， and low-dose， medium-dose， high-dose experimental group (perfused with 2.5×106， 5×106， 1×107 CFU/0.4 mL composite probiotics) for 28 days. The viscera index， cellular immune function， humoral immune function， percentage of splenic lymphocyte subsets， serum immunoglobulin and cytokine levels were determined. The results showed that， compared with model group， the thymus index of mice in each dose group was significantly increased (P<0.01)， and that in the low， medium and high dose groups was increased by 58.92%， 67.86% and 80.36%. The degree of delayed type allergic reaction increased (P<0.01) 20.34%， 27.12% and 67.80% in low， medium and high dose groups. Serum hemolysin levels were increased in both groups (P<0.05)， and and were significantly increased in medium dose and high dose groups (P<0.01)， by 31.25% and 40.23%， respectively. The percentages of CD3+， CD4+CD8- and CD4-CD8+ in splenic lymphocytes increased in low， medium and high dose groups (P<0.05)， and CD3+ was the most significant (P<0.01)， while those in low， medium and high dose groups increased by 33.17%， 42.63% and 54.65%， respectively. The proportion of natural killer (NK) cells in high-dose group was significantly increased by 56.50% (P<0.01)， and serum levels of immunoglobulin IgA， IgG， IL-2 and TNF-α were increased (P<0.05). In conclusion， compound probiotics could significantly improve the immunity of CTX mice， providing an important reference for the development of health food to enhance the immunity.