In this study， the antioxidant ingredients of Lactobacillus brevis AR247 were explored， then anti-aging effects of L. brevis AR247 on D-galactose induced aging mice were measured. The aqueous phase and intracellular constituents of L. brevis AR247 showed higher antioxidant activity than others. The aging mice model was established by injecting of D-galactose on the concentration of 150 mg/（kg·d） for 6 weeks. These mice were simultaneously administrated by gavage with L. brevis AR247 on the concentration of 1.0×109 CFU/mL （1 mL/100 g）. After for 6 weeks， the activities of catalase （CAT）， superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）， and the contents of glutathione （GSH） and malondialdehyde （MDA） in serum， liver and brain were measured by commercial reagent kits. The contents of Interleukin 2 （IL-2） and tumour necrosis factor-α （TNF-α） in serum were also determined. The results showed that L. brevis AR247 had antagonism effects on D-galactose-induced aging mice such as recovered body weight and brain index to normal levels， and it could increase the activities of CAT， SOD and GSH-Px （P<0.05）， the content of GSH （P<0.05）， and reduce MDA levels （P<0.05） such as reduce the content of MDA in liver to 66.16% of normal group. Compared with the aging model group， the content of serum IL-2 in L. brevis AR247 group was significantly increased （P<0.05）， and it increased to 104.50% of normal group. In conclusion， L. brevis AR247 showed antioxidant and anti-aging effects. The underlying mechanism might be related to improvement of antioxidant function and immune function in the body.