酪蛋白凝胶颗粒对水包油型乳液稳定性及体外消化的影响
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国家自然科学基金项目(31901625)


The Stability and in Vitro Digestion of Oil-in-water Emulsions Stabilized by Casein Gel Particles
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    摘要:

    研究酪蛋白凝胶颗粒对水包油型乳液的稳定性及体外消化特性的影响,并与商业乳化剂酪蛋白酸钠和吐温20作对比。采用马尔文激光粒度仪、纳米粒度电位仪及稳定性分析仪分别测定水包油乳液的粒径、电位和稳定性;通过模拟体外消化,研究酪蛋白凝胶颗粒稳定的乳液的消化情况并比较不同种类乳化剂对乳液体外消化特性的影响。结果表明,酪蛋白凝胶颗粒稳定的乳液不稳定性指数为0.71±0.09,显著低于酪蛋白酸钠和吐温稳定乳液的不稳定性指数(分别为1.43±0.09,1.62±0.15),稳定性最好。这与酪蛋白软颗粒能以完整的颗粒结构存在于油水界面后形成较强的空间位阻和静电作用有关。体外消化结果表明,酪蛋白凝胶颗粒稳定的乳液脂肪酸的释放量为(86.13±3.91)%,而酪蛋白酸钠和吐温20稳定的乳液脂肪酸释放量分别为(86.79±3.61)%,(91.62±1.31)%,三者并没有显著性差异,表明酪蛋白凝胶颗粒对乳液消化不产生抑制作用。乳化剂种类虽对初始消化速率有影响,但不影响消化终点。结论:酪蛋白凝胶颗粒能够较好地稳定水包油乳液且不影响其消化。

    Abstract:

    This article aimed to investigate the stability and the in vitro digestion characteristics of the oil-in-water emulsions stabilized by casein gel particles, comparing with sodium caseinate and tween 20. The droplet size, zeta potential and stability of oil-in-water emulsions were measured using Mastersizer, Zetasizer and Turbiscan lab, respectively. The digestion characteristics of the emulsions stabilized by casein gel particles were investigated by in vitro simulated digestion, making comparison to sodium caseinate and tween 20. The results showed that the Turbiscan stability index of oil-in-water emulsions stabilized by casein gel particles was 0.71 ± 0.09, which was significantly lower than the emulsions stabilized by sodium caseinate (1.43 ± 0.09) and tween 20 (1.62 ± 0.15), indicating that the stability of the casein gel particles stabilized emulsions was best. This may be attributed to strong steric hindrance and electrostatic repulsion formed by casein gel particles with complete structure on the oil-water interface. The results of in vitro digestion showed that the release of the free fatty acid of the emulsions stabilized by casein gel particles, sodium caseinate and tween 20 was(86.13 ± 3.91)%, (86.79 ± 3.61)% and (91.62 ± 1.31)%, respectively. There was no significant difference in lipolysis among three emulsions, indicating that casein gel particles would not impede lipid digestion of the emulsions. The type of emulsifiers had effects on the initial rate of lipid digestion, but no effects on the final lipid digestion. In conclusion, casein gel particles can improve the stability of oil-in-water emulsions and have no effect on the digestion.

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陈冲;张宁;任怡镁;王鹏杰.酪蛋白凝胶颗粒对水包油型乳液稳定性及体外消化的影响[J].中国食品学报,2020,20(9):30-37

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  • 在线发布日期: 2020-10-13
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