柑橘囊衣果胶多糖及其降解产物硫酸酯化对抗肿瘤活性的影响
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(1.浙江大学生物系统工程与食品科学学院 智能食品加工技术与装备国家地方联合工程实验室浙江省农产品加工技术研究重点实验室 南方果蔬保鲜技术集成科研基地浙江省健康食品制造与品质控制国际合作基地 杭州 310058;2.浙江大学馥莉食品研究院 杭州 310058;3.浙江大学宁波研究院 浙江宁波 315100)

作者简介:

朱凯(1992—),男,硕士

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基金项目:

国家自然科学基金面上项目(31871815);浙江省重大专项(2020C02033)


Effects of Sulfation of Pectin Polysaccharides and Its Degraution Products from Citrus Segment Membrane on Antitumor Activity
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(1.College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Integrated Research Base of Southern Fruit and Vegetable Preservation Technology, Zhejiang International Scientific and Technological Cooperation Base of Health Food Manufacturing and Quality Control, Zhejiang University, Hangzhou 310058;2.Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058;3.Ningbo Research Institute, Zhejiang Universizty, Ningbo 315100, Zhejiang)

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    摘要:

    果胶是广泛存在于植物细胞壁中的大分子多糖,具有多种生理活性功能。有研究发现对多糖进行改性,可以提高其生物活性。本文以柑橘罐头废水中回收的柑橘囊衣果胶(PP)及其降解寡糖(POS1和POS2)为研究对象,通过硫代巴比妥酸(TBA)和二甲基亚砜(DMSO)两种方法进行硫酸酯化,提高其活性。分子质量和单糖组成分析表明:经两种体系硫酸酯化后,果胶多糖和寡糖分子质量均降低,且均有阿拉伯糖和半乳糖单糖单元脱落。采用傅里叶变换红外光谱(FTIR)、核磁共振谱(NMR)对柑橘囊衣果胶多糖及其硫酸酯产物进行结构分析,各组分的半乳糖醛酸的H4在硫酸酯化后出现低场位移动,POS1的半乳糖异头氢和POS2的阿拉伯糖异头氢信号均向低场位移动,说明在主链和侧链均发生硫酸酯化。细胞模型试验表明,对柑橘囊衣果胶寡糖POS1及POS2进行硫酸酯化后,其体外抗肿瘤活性增强。其中,POS1-T的效果最佳,为30.25%。这说明硫酸化可有效提高果胶的抗肿瘤效果。

    Abstract:

    Pectin is a polysaccharide that widely exists in plant cell walls and has a variety of physiologically active functions. Various studies have confirmed that modification of polysaccharide can improve its biological activity. Therefore, in this study, the citrus segment membrane pectin (PP) and its degraded oligosaccharides (POS1 and POS2) recovered from the citrus canning waste water were carried out sulfate esterification in order to increase their activity. Molecular weight and monosaccharide composition analysis showed that after sulfating by TBA and DMSO systems, the molecular weight of sulfated pectin polysaccharides and oligosaccharides were decreased, and the content of arabinose and galactose reduced significantly. FTIR and NMR results shown that the H4 of each component of galacturonic acid showed a low-field shift after sulfation. And the anomer hydrogen signals of galactose and arabinose from POS1 and POS2 shifted to the low field, respectively. Which indicating that the main chain and the side chain were sulfated. The in vitro results showed that the anti-tumor activity of sulfated POS1 and POS2 was further enhanced. Among them, POS1-T has the best effect, with an inhibition rate of 30.25%. In summary, the results of the study indicated that sulfation could increase the antitumor effect of pectin polysaccharide and its oligosaccharides.

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朱凯,刘闪闪,叶兴乾,陈士国.柑橘囊衣果胶多糖及其降解产物硫酸酯化对抗肿瘤活性的影响[J].中国食品学报,2022,22(2):23-30

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  • 收稿日期:2021-02-21
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  • 在线发布日期: 2022-03-03
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