The stable nanoemulsion can be prepared by high-pressure homogenization and ultrasonic. The nanoemulsion prepared by high pressure homogenization is more stable， and has smaller average particle size， smaller PDI value， lower turbidity， lower yield， lower TSI and higher Emulsification yield， higher β-carotene entrapment efficiency than ultrasonic prepared. The composition of the interface adsorption protein subunits of the two kinds of nanoemulsions is close， which is composed of the 11 S globulin basic subunit with molecular weight of 20 ku and the 11 S globulin acidic subunit with molecular weight of 35 ku. The interfacial protein content of nano emulsion prepared by ultrasonic was higher than high-pressure homogenization prepared， but the nanoemulsion prepared by ultrasonic has the lower viscosity than high-pressure homogenization prepared.