In order to study the dominant spoilage bacteria and their spoilage ability of Lateolabrax japonicus fillets during chilled storage at the end of storage， the specific colonies of Lateolabrax japonicus were obtained by selective mediums. The dominant spoilage bacteria were determined according to results of the selective mediums combined with sensory evaluation. The pure strains were isolated by plate streaking and the species were screened by the observation of colony morphology. The genus and species of bacteria was analyzed by physiological and biochemical experiments， 16S rDNA technique. The identified dominant spoilage bacteria were inoculated on Lateolabrax japonicus with sterile treated and stored at 4 ℃ under cold storage. Total volatile base nitrogen （TVB-N） and total viable counts on different storage periods were measured respectively， TVB-N yield factor （YTVB-N） was used to evaluate the spoilage ability of bacteria. The results show that five strains of Pseudomonas sp were screened including P1（Pseudomonas sp. WCS374） and P2 （Pseudomonas fragi P121）， P3 （Pseudomonas fragi B-727）， S1 （Putrefaciens Shewanella） and S2 （Shewanella baltica OS185）. According to the results of spoilage capability， their order as follows： S1>S2>P2>P3>P1. The spoilage ability of P2 （Pseudomonas fragi） is higher than that of P1 （Pseudomonas sp. WCS374） and P3 （Pseudomonas fragi B-727）， Shewanella. sp was higher than that of Pseudomonas sp.