Snail’s mucus contains abundant natural collagen， elastin， urine membrane element， protein， vitamins， glycolic acid. In this paper， the snail secretion was extracted by method of NaCl stimulus. The contents of crude protein in the mucus were determined by the Kjeldah Method. The results show that the crude protein of the mucus was about 0.42% in fresh snail mucus， while crude protein content was 31.48% in freeze-dried samples. The collagen of mucus was measured by the UV pectrophotometer and the presence of type I collagen in snail mucus was proved by Fourier-transform infrared spectroscopy （FT-IR）. The content of collagen determined by Woessner method was about 16.69%. HPLC separation was developed for the determination of simultaneous quantitative analysis of allantoin and glycolic acid in snail mucus. Finally， it was found that snail mucus had bacteriostatic activity against Shigella， Salmonella suis， Pasteurella avium and Staphylococcus aureus. This study will contribute to the research in the field of food， cosmetics and pharmaceutical and application.