Botrytis cinereais the main pathogenic fungi of post-harvested blueberry. The enzyme production condition of Botrytis cinerea was optimized by signal factor analysis and its enzymatic properties were studied. Results showed that the maximum lipase production was obtained after 96 h fermentation of medium contained 1%（m/m） of olive oil and 1% of peptone. Results of enzymaticproperties showed that this lipase had a wide adaptation to pH and temperature. The hydrolytic activity of crude lipase was highest when reaction temperature was 40 ℃ and pH = 9.0. The activity of crude lipase was activated by Na+ and Fe3+， but was inhibited by K+，Ca2+，Mg2+ and Cu2+. The crude lipase had good tolerance to isopropyl alcohol， methanol and acetone， and ethyl acetate and benzeneenhanced its activity. Optimizing the fermentationconditions of Botrytis cinerea could increase the production of lipase and lay the foundation for the furtherisolation and purification of lipases. Results of this study will provide a guidance on pre-harvest disease control and post-harvest quality protection of blueberries.