基于近红外光谱结合代谢流分析的L-缬氨酸发酵过程监控
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国家自然科学基金项目(31470211);工业微生物优良菌种选育与发酵技术公共服务平台项目(17PTGCCX00190)


Fermentation Process Monitoring of L-valine Based on Near Infrared Spectroscopy Combined with Metabolic Flow Analysis
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    摘要:

    L-缬氨酸作为必需氨基酸之一,在许多发酵类食品配料方面有重要应用。及时监控其发酵过程,有利于L-缬氨酸产品品质的提升。本文建立了一种发酵类的食品配料生产过程监控新方法。该方法通过对发酵原料和产物进行分析,建立主要原料及产物的近红外模型,结合代谢流模型,能够快速分析不同发酵条件下细胞内部代谢流分布及变化,实时监测发酵过程各项参数。该方法能够实现同时对多组分快速、准确的检测,从而弥补了传统检测方法的缺点和不足;能够快速分析获得发酵过程细胞内部代谢流分布,从而帮助控制发酵过程条件,实时监测发酵过程参数,为发酵过程自动化控制提供理论基础,为发酵类食品配料生产过程监控提供方法。

    Abstract:

    L-valine, as one of the essential amino acids, has important applications in many fermented food ingredients. Timely understanding and monitoring its fermentation process is beneficial to improve the quality of L-valine products. In this paper, a new method for monitoring the production process of fermented food ingredients was developed. And the near infrared model of the main raw materials and products was established by analyzing the raw materials and products of fermentation. On this basis, combined with the metabolic flux model, the distribution and variation of metabolic flux in cells under different fermentation conditions can be rapidly analyzed monitor the parameters of the fermentation process in real time. The method can simultaneously detect the components quickly and accurately, thus making up for the shortcomings and shortcomings of the traditional detection methods; Meanwhile the method can quickly and accurately obtain the metabolic flow distribution in the cell during fermentation, so as to help control the fermentation process, monitoring the fermentation process parameters for real-time. It provides a theoretical basis for the automatic control of fermentation process, and provides a method for monitoring the production process of fermented food ingredients.

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魏洪波;石 拓;崔 毅;张权威;杜丽红;陈启欣;谢希贤;陈 宁.基于近红外光谱结合代谢流分析的L-缬氨酸发酵过程监控[J].中国食品学报,2019,19(11):79-87

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  • 在线发布日期: 2019-12-02
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