In order to separate and breed the main lactic acid bacteria from Yunnan Jianshui fermented soybean whey tofu， and pure breed ferment soybean whey， the isolation and identification of lactic acid bacteria in the coagulant of fermented soybean whey which was used in the production of Yunnan Jianshui fermented soybean whey tofu were carried out. Five strains of lactic acid bacteria were isolated and purified by plate streak. The strains were identified by morphological observation， physiological and biochemical characteristics and 16S rDNA sequences. The results showed that SYG01-SYG05 respectively was Lactobacillus brevis， Lactobacillus coryniformis， Lactobacillus curvatus， Weissella confusa and Lactobacillus concavus. The study not only provided a new source of resources for developing new probiotics fermented soybean products， but also provided theoretical basis for the preparation of standardized fermented soybean whey and scientific technical specifications for making fermented soybean whey tofu， and had great significance to the large-scale production and promotion of fermented soybean whey tofu.