沙蚕EN制剂改善肝癌前病变小鼠代谢的研究
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国家自然科学基金项目(81502955);上海市科技创新行动计划(15410722500)


Aibuhitensis EN Formula for Improving Metabolic Characteristics of Liver Precancerous Lesions in Mice
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    摘要:

    目的:研究双齿围沙蚕肠内营养制剂对肝癌前病变小鼠营养代谢和炎症反应的影响。方法:用二乙基亚硝胺诱发和制备早期肝癌前病变小鼠模型,设置沙蚕处理组1(A)、沙蚕处理组2(B)、沙蚕处理组3(C)、阳性对照组(D)、空白组(E)和对照组(F)。分别在处理D7、D14、D21和D28采血取样,检测血浆营养指标、炎症指标、肝功能指标,最后观察肝脏病理变化。结果:双齿围沙蚕肠内营养制剂处理期间,小鼠体重逐渐增加,D28,A组比E组体重增加61.49%;A组的TP、Alb含量都显著高于E组但低于F组,D28,A组分别增加30.32%和13.68%。D7、D14、D21和D28,A组的TNF-α水平显著低于E,D28,A组比E组减少30.15%;D28,A组比E组的IL-2显著升高27.77%。D7、D14、D21和D28,A组的ALT和AST水平与E组相比显著降低,D28,A组分别降低83.75%和128.20%;A组与E组相比具有良好的肝组织形态学特征。结论:以双齿围沙蚕为蛋白源的肠内营养制剂作为特殊医学用途配方食品,能促进肝癌前病变实验动物体内合成TP和Alb等蛋白质,改善早期肝癌前病变小鼠的营养代谢状况,缓解肝癌前病变实验动物TNF-α、IL-2等炎症反应,减轻ALT和AST反映出的肝癌前病变实验动物肝组织形态学受损。当沙蚕蛋白含量为肠内营养制剂蛋白总量的60%时,双齿围沙蚕肠内营养制剂营养功效显著。

    Abstract:

    Objective: To investigate the effects of enteral nutrition in Perinereis aibuhitensis on nutrition metabolism and inflammation of mice with precancerous lesions. Methods: The mice model of precancerous lesions were induced and prepared with diethylnitrosamine, and then clamworm treatment group 1(A), clamworm treatment group 2(B), clamworm treatment group 3 (C), a positive control group (D), a blank group (E) and a control group (F) were set up. The plasma nutrition index, inflammation index and liver function index were detected after the blood sampling of D7, D14, D21 and D28 respectively. Finally, the hepatic pathological changes were observed. Result: The weight of mice increased gradually during the treatment of enteral nutrition in Perinereis aibuhitensis. For D28, the weight of group A was 61.49% higher than group E; The contents of TP and Alb of group A were significantly higher than group E, while lower than F group. Group A increased by 30.32% and 13.68% separately. For D7, D14, D21 and D28, the TNF-α level of group A was obviously lower than group E. For D28, group A decreased by 30.15% when compared with group E; Group A had a significantly higher level of IL-2 than group E. For D7, D14, D21 and D28, the ALT and AST levels of group A markedly decreased compared with group E. For D 28, group A were 83.75% and 128.20% lower respectively. Group A had good histological features of liver tissue compared with group E. Conclusion: When Perinereis aibuhitensis was used as protein source of enteral nutrition, the special medical purpose formula can promote the synthesis of TP and Alb in the liver of precancerous lesions of experimental animals, and improve the nutritional metabolism of liver precancerous lesions in mice. It can also relieve the inflammation of TNF-α, IL-2 in liver precancerous lesions experimental animals, as well as the liver tissue morphological damage reflected by ALT and AST. When the protein content of silkworm was 60% of the total protein of enteral nutrition, the nutritional effect of the Perinereis aibuhitensis enteral nutrition formula was significant.

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李慧婷;李娓;郭锐华;费迎心;吴文惠;包斌.沙蚕EN制剂改善肝癌前病变小鼠代谢的研究[J].中国食品学报,2019,19(12):22-30

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  • 在线发布日期: 2020-01-09
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