Objective： To evaluated the regulatory effects of zeaxanthin on lipid and energy metabolism in obese mice. Methods： The obese mice were fed with or without zeaxanthin（40 and 20 mg/kg） for 4weeks， and the body weight， masses of total adipose and liver tissues were detected； Morphological status of liver was observed through HE staining， and the mRNA expression involved in lipid and energy metabolism of liver including CCAAT enhancer binding protein alpha （C/EBPα）， Sterol-regulatory Element-binding Protein-1c（SREBP1-c）， Acetyl-CoA carboxylase （ACC）， fatty acid translocase （CD36）， peroxlsome proliferator-activated receptor-γ coactlvator-1α （PGC-1α）， uncoupling protein 1 （UCP1） and Silence information regulator T1 （SIRT1） as well as AMPK signaling pathway were investigated. Results： Compared with the HFD group， zeaxanthin significantly decreased the body weight gain， liver mass， down-regulated the expression of C/EBPα， SREBP1-c， ACC， CD36 and up-regulated UCP1. What’s more， low dose of zeaxanthin treatment reversed the high-fat diet-induced inhibition of Adenosine 5’-monophosphate（AMP）-activated protein kinase （AMPK） phosphorylation in hepatic tissue and alleviated fatty degeneration of liver. Conclusion： Zeaxanthin inhibited the hepatic lipid accumulation and improved the pathological state in high-fat diet induced obese mice by activated the AMPK pathway and down-regulated the expression of C/EBPα， SREBP1-c， ACC， CD36 and up-regulated UCP1 in liver.