葡聚糖及其糖基化修饰对白鲢鱼糜凝胶特性的影响
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湖北省自然科学基金项目(2015CFB389);国家现代农业产业技术体系专项基金(CARS-45-27)


Effect of Dextran and Glycosylation on Gel Properties of Surimi from Silver Carp (Hypophthalmichthys molitrix)
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    摘要:

    以鲢鱼为对象,研究糖基化反应时间和比例(蛋白与葡聚糖质量比)对鲢鱼鱼糜凝胶特性及微观结构的影响。结果显示:未添加葡聚糖的鱼糜凝胶的pH以及420 nm和294 nm处的吸光值随着加热时间的延长没有显著变化(P>0.05),而添加了葡聚糖的鱼糜凝胶的pH值显著降低(P<0.05),420 nm和294 nm处的吸光值也逐渐增加,说明在40 ℃加热过程中,添加葡聚糖的鱼糜凝胶发生了糖基化反应,且随着葡聚糖的增多,糖基化反应程度逐渐增大。未经过糖基化反应的鱼糜凝胶(CK组)的凝胶强度和网络致密度在加热的前4 h内显著高于经糖基化反应的鱼糜凝胶。CK组的凝胶强度和微观网络致密度随着加热时间的延长而显著降低(P<0.05),而经糖基化反应的鱼糜凝胶的强度及微观结构变化不显著(P>0.05)。当葡聚糖添加量越多,鱼糜凝胶的特性越弱。葡聚糖在白鲢鱼糜加热过程中与蛋白发生糖基化反应,添加葡聚糖虽降低了鱼糜凝胶的特性,但增强了凝胶在加热过程中的稳定性。

    Abstract:

    We investigated effect of reaction time and ratio (protein and dextran weight ratio) of glycosylation on gel properties of surimi from silver carp by texture analyzer and scanning electron microscope. The results showed that pH value, absorptions of 420 nm and 294 nm of surimi gel without dextran had no significant change during heating(P>0.05). However, the pH value of surimi gel with dextran were decreased significantly(P<0.05), and absorptions of 420 nm and 294 nm of surimi gel with dextran were increased gradually. This indicated that there were glycosylation between dextran and surimi proteins during 40 ℃ heating, and the degree of glycosylation was increased with the dextran ratio increase. In the first 4 h heating, gel strength and network density of surimi gel without dextran was higher than that of surimi gels with dextran. And gel strength and network density of surimi gel without dextran declined significantly with the heating time increasing(P<0.05), but both that properties of surimi gel with dextran had no significantly change(P>0.05). Moreover, with degree of glycosylation increase, gel properties of surimi gels were decrease. Therefore, glycosylation occurred when adding dextran to surimi during heating. And glycosylation between dextran and surimi proteins decreased the gel properties of surimi gel from silver carp, but it increased the heat stability of surimi gel during heating processing.

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尤娟;姜雪英;尹涛;胡杨;黄琪琳;熊善柏.葡聚糖及其糖基化修饰对白鲢鱼糜凝胶特性的影响[J].中国食品学报,2019,19(12):69-75

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  • 在线发布日期: 2020-01-09
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