In this study， the β-carotene-coix seed oil complex liposome was prepared， and the optimal preparation conditions of the complex liposome are optimized by the Box-Behnken response surface method based on the single factor experiment. The internal structure and morphology of the samples were observed by transmission electron microscopy （TEM）. The results showed the optimal preparation conditions as follows： temperature was at 43 ℃， mass ratio of lecithin to cholesterol was 7 ∶ 1， volume of PBS buffer was 25 mL， and under this condition， the entrapment efficiency of β-carotene was 84.39%， The average particle size was 187.9 nm ± 7.5 nm， the polydispersity coefficient （PDI） was 0.104 ± 0.09， and the average Zeta potential was -29.33 mV ± 4.77 mV. The results of TEM showed that the complex liposome were more homogeneous than the β-carotene liposome， and the DPPH free radical scavenging ability of the complex liposome was higher than that of β-carotene liposome and coix seed oil liposome.