This article used transcriptomic analysis and bioinformatics analysis to study the differentially expressed genes at the transcriptional level after combining linoleic acid-induced human umbilical vein endothelial cells. The results showed that 126 genes were significantly changed， 28 of them were up-regulated and 98 genes were down-regulated. And the bioinformatics analysis showed that there were 54 significant differences in the gene expression of the cells， and 118 genes were significantly different from those of the gene. In addition， a total of 99 signaling pathways were detected， of which 7 pathways were significantly changed， including terpenoid skeletal biosynthetic pathways， steroid biosynthetic pathways and TNF signaling pathways. Finally， according to the PPI gene interaction pathway map， the target genes involved in different pathways were analyzed， including matrix metalloproteinase-3， FOS and TNF receptor associated factor-1.